一种基于二维偏振干涉测量法的平行扫描角表面等离子体共振生物传感器。

A two-dimensional polarization interferometry based parallel scan angular surface plasmon resonance biosensor.

作者信息

Liu Le, Ma Suihua, Ji Yanhong, Chong Xinyuan, Liu Zhiyi, He Yonghong, Guo Jihua

机构信息

Laboratory of Optical Imaging and Sensing, Graduate School at Shenzhen, Tsinghua University, Shenzhen, China.

出版信息

Rev Sci Instrum. 2011 Feb;82(2):023109. doi: 10.1063/1.3553028.

DOI:10.1063/1.3553028

PMID:21361575

Abstract

We describe a two-dimensional polarization interferometry based parallel scan angular surface plasmon resonance (SPR) biosensing technique. The method of line-shaped light illumination and parallel scan offers a high throughput. The simultaneous record of SPR angular spectrum enables the system to be unaffected by the time-dependent variation of the light source. The polarization interferometry technique lowers the minimum of the SPR dip and thereby reduces the noise related to the light intensity. Refractive index resolutions of 1.4 × 10(-6) refractive index unit (RIU) under normal condition and 4.6 × 10(-7) RIU under a more time-consuming condition are achieved in our angle interrogation based sensor. Meanwhile, a manually prepared DNA microarray has been detected, showing the potential applications of this technique in microarray analysis.

摘要

我们描述了一种基于二维偏振干涉测量的平行扫描角表面等离子体共振（SPR）生物传感技术。线形光照和平行扫描方法提供了高吞吐量。SPR角谱的同时记录使系统不受光源随时间变化的影响。偏振干涉测量技术降低了SPR凹陷的最小值，从而降低了与光强度相关的噪声。在我们基于角度询问的传感器中，正常条件下折射率分辨率达到1.4×10^(-6) 折射率单位（RIU），在更耗时的条件下达到4.6×10^(-7) RIU。同时，已检测了手动制备的DNA微阵列，表明该技术在微阵列分析中的潜在应用。