Zeng Youjun, Wang Lei, Wu Shu-Yuen, He Jianan, Qu Junle, Li Xuejin, Ho Ho-Pui, Gu Dayong, Gao Bruce Zhi, Shao Yonghong
College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen Key Laboratory of Sensor Technology, Shenzhen University, Shenzhen 518060, China.
Department of Electronic Engineering, The Chinese University of Hong Kong, Shatin 999077, Hong Kong, China.
Sensors (Basel). 2017 Jan 5;17(1):90. doi: 10.3390/s17010090.
A fast surface plasmon resonance (SPR) imaging biosensor system based on wavelength interrogation using an acousto-optic tunable filter (AOTF) and a white light laser is presented. The system combines the merits of a wide-dynamic detection range and high sensitivity offered by the spectral approach with multiplexed high-throughput data collection and a two-dimensional (2D) biosensor array. The key feature is the use of AOTF to realize wavelength scan from a white laser source and thus to achieve fast tracking of the SPR dip movement caused by target molecules binding to the sensor surface. Experimental results show that the system is capable of completing a SPR dip measurement within 0.35 s. To the best of our knowledge, this is the fastest time ever reported in the literature for imaging spectral interrogation. Based on a spectral window with a width of approximately 100 nm, a dynamic detection range and resolution of 4.63 × 10 refractive index unit (RIU) and 1.27 × 10 RIU achieved in a 2D-array sensor is reported here. The spectral SPR imaging sensor scheme has the capability of performing fast high-throughput detection of biomolecular interactions from 2D sensor arrays. The design has no mechanical moving parts, thus making the scheme completely solid-state.
本文介绍了一种基于波长询问的快速表面等离子体共振（SPR）成像生物传感器系统，该系统使用声光可调滤波器（AOTF）和白光激光器。该系统结合了光谱方法提供的宽动态检测范围和高灵敏度的优点，以及多路复用高通量数据采集和二维（2D）生物传感器阵列。其关键特性是利用AOTF实现来自白色激光源的波长扫描，从而快速跟踪由目标分子与传感器表面结合引起的SPR凹陷移动。实验结果表明，该系统能够在0.35秒内完成一次SPR凹陷测量。据我们所知，这是文献中报道的用于成像光谱询问的最快时间。在此报告了基于宽度约为100 nm的光谱窗口，在二维阵列传感器中实现的动态检测范围和分辨率分别为4.63×10折射率单位（RIU）和1.27×10 RIU。光谱SPR成像传感器方案具有对二维传感器阵列中的生物分子相互作用进行快速高通量检测的能力。该设计没有机械运动部件，因此使该方案完全是固态的。
