视频速率宽场相干反斯托克斯拉曼散射显微镜，采用共线非相位匹配照明。

Video-rate wide-field coherent anti-Stokes Raman scattering microscopy with collinear nonphase-matching illumination.

机构信息

University of Konstanz, Department of Chemistry, D-78457 Konstanz, Germany.

出版信息

J Biomed Opt. 2011 Feb;16(2):021102. doi: 10.1117/1.3533707.

Abstract

A simple scheme for video-rate wide-field coherent anti-Stokes Raman scattering (CARS) microscopy is presented. The method is based on collinear nonphase-matching illumination. The mechanisms leading to CARS signal generation are investigated. We find that refraction-mediated phase-matching is the main effect. Video-rate wide-field CARS microscopy of polystyrene beads and CARS wide-field images of C. elegans embryos are shown, and the capabilities and the limitations of the scheme are discussed.

摘要

提出了一种用于视频速率宽场相干反斯托克斯拉曼散射（CARS）显微镜的简单方案。该方法基于共线非相位匹配照明。研究了产生 CARS 信号的机制。我们发现折射介导的相位匹配是主要效应。展示了聚苯乙烯珠的视频速率宽场 CARS 显微镜和 C. elegans 胚胎的 CARS 宽场图像，并讨论了该方案的能力和局限性。