Aix-Marseille Université, CNRS, Institut Fresnel, Ecole Centrale Marseille, Campus de Saint Jéro^me, Marseille, 13397 Cedex 20, France.
J Biomed Opt. 2011 Feb;16(2):021106. doi: 10.1117/1.3533311.
We present the assets and constraints of using optical parametric oscillators (OPOs) to perform point scanning nonlinear microscopy and spectroscopy with special emphasis on coherent Raman spectroscopy. The different possible configurations starting with one OPO and two OPOs are described in detail and with comments that are intended to be practically useful for the user. Explicit examples on test samples such as nonlinear organic crystal, polystyrene beads, and fresh mouse tissues are given. Special emphasis is given to background-free coherent Raman anti-Stokes scattering (CARS) imaging, including CARS hyperspectral imaging in a fully automated mode with commercial OPOs.
我们介绍了使用光学参量振荡器（OPO）进行点扫描非线性显微镜和光谱学的优点和限制，特别强调了相干拉曼光谱学。详细描述了从一个 OPO 到两个 OPO 的不同可能配置，并提供了旨在对用户实用的注释。还给出了非线性有机晶体、聚苯乙烯珠和新鲜小鼠组织等测试样品的示例。特别强调了无背景相干拉曼反斯托克斯散射（CARS）成像，包括使用商用 OPO 以全自动模式进行 CARS 高光谱成像。
