An Gang, Li Cheng-Wen, Li Qian, Yi Shu-Hua, Liu Xu-Ping, Xu Yan, Li Zeng-Jun, Qi Jun-Yuan, Zou De-Hui, Qiu Lu-Gui
Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences, Tianjin 300020, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2011 Feb;19(1):54-8.
This study was to aimed investigate the influence of immunomagnetic sorting on detecting the genetic aberrations of multiple myeloma (MM) by interphase fluorescence in situ hybridization (FISH) and to explore the detection method suitable to use in our country. The genetic aberrations of immunomagnetically sorted and unsorted bone marrow cells from the same MM patients were detected by interphase FISH and the detectable rate of genetic aberration was compared. The types of probes included 13 q14 (RB-1) and 14q32 (IGH). The 42 and 22 sorted and unsorted marrow samples from MM patients were detected by using 13q14 probe and 14q32 probes respectively, the results indicated that the 13q14 deletion was found in 9 of 42 (21.4%) unsorted marrow samples and in 25 of 42 (56.8%) CD138(+)-sorted marrow samples. The 13q32 rearrangement was found in 7 of 22 (31.8%) unsorted marrow samples and in 14 of 22(63.6%) CD138(+)-sorted marrow samples. Both of the difference was statistically significant (p = 0.001 and p = 0.035 respectively). Percentages of cytogenetic alterations detected in unsorted bone marrow cells correlated positively with percentage of plasma cells tested by bone marrow smears or flow cytometry. When percentage of plasma cells tested by bone marrow smears exceed 50%, or by flow cytometry exceed 10%, there was no difference between 2 methods. It is concluded that immunomagnetic sorting of CD138(+) cells increases the probability of detection of the 13q14 deletion and 14q32 rearrangement in bone marrow samples. The low detectable rate of genetic aberration in unsorted bone marrow cells is associated to the low percentage of plasma cells in bone marrow samples, higher percentage of plasma cells can partly overcome the shortage of unsorted detection method. When percentage of plasma cells tested by bone marrow smears exceed 50%, or by flow cytometry exceed 10%, there was no difference between 2 methods.
本研究旨在探讨免疫磁珠分选对间期荧光原位杂交（FISH）检测多发性骨髓瘤（MM）基因畸变的影响，并探索适合我国使用的检测方法。通过间期FISH检测同一MM患者免疫磁珠分选和未分选的骨髓细胞的基因畸变情况，并比较基因畸变的可检测率。所用探针类型包括13q14（RB-1）和14q32（IGH）。分别用13q14探针和14q32探针检测42例和22例MM患者分选和未分选的骨髓样本，结果显示，42例未分选骨髓样本中有9例（21.4%）检测到13q14缺失，42例CD138（+）分选骨髓样本中有25例（56.8%）检测到13q14缺失。22例未分选骨髓样本中有7例（31.8%）检测到14q32重排，22例CD138（+）分选骨髓样本中有14例（63.6%）检测到14q32重排。两者差异均有统计学意义（分别为p = 0.001和p = 0.035）。未分选骨髓细胞中检测到的细胞遗传学改变百分比与骨髓涂片或流式细胞术检测的浆细胞百分比呈正相关。当骨髓涂片检测的浆细胞百分比超过50%，或流式细胞术检测的浆细胞百分比超过10%时，两种方法之间无差异。结论是，CD138（+）细胞的免疫磁珠分选增加了骨髓样本中13q14缺失和14q32重排的检测概率。未分选骨髓细胞基因畸变的可检测率低与骨髓样本中浆细胞百分比低有关，较高的浆细胞百分比可部分克服未分选检测方法的不足。当骨髓涂片检测的浆细胞百分比超过50%，或流式细胞术检测的浆细胞百分比超过10%时，两种方法之间无差异。
