Hu Hui-Jin, Lu Hua, Fei Xiao-Ming, Li Jun-Xia, Li Jian-Yong
Department of Hematology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2011 Feb;19(1):59-63.
This study was aimed to investigate the mRNA expression levels of hepatocyte growth factor (HGF), stromal cell-derived factor-1 (SDF-1), monocyte chemotactic protein-1 (MCP-1) and interleukin-8 (IL-8) in bone marrow mesenchymal stem cells (MSC) from multiple myeloma (MM) patients. The mRNA expression levels of HGF, SDF-1, MCP-1 and IL-8 in bone marrow MSC from 20 newly diagnosed MM patients were detected by real time quantitative RT-PCR and were compared with that in 9 controls. The results indicated that the mean mRNA expression level of HGF was up-regulated in MM patients, as compared with controls (p < 0.01). However, the mean mRNA expression level of SDF-1 mRNA was down-regulated in MM patients, as compared with controls (p < 0.05). There was no significant difference in the mRNA expression levels of MCP-1 and IL-8 between MM and control cohorts (p > 0.05). It is concluded that BM-MSC from MM patients express HGF, SDF-1, MCP-1, IL-8, but these chemotaxis-related factors expression of bone marrow microenvironment cellular component are dysregulated in MM patients, which may result from the interplay between MM cells and MSC.
本研究旨在探讨多发性骨髓瘤（MM）患者骨髓间充质干细胞（MSC）中肝细胞生长因子（HGF）、基质细胞衍生因子-1（SDF-1）、单核细胞趋化蛋白-1（MCP-1）和白细胞介素-8（IL-8）的mRNA表达水平。采用实时定量逆转录聚合酶链反应（RT-PCR）检测20例新诊断MM患者骨髓MSC中HGF、SDF-1、MCP-1和IL-8的mRNA表达水平，并与9例对照者进行比较。结果显示，与对照组相比，MM患者HGF的平均mRNA表达水平上调（p<0.01）。然而，与对照组相比，MM患者SDF-1 mRNA的平均表达水平下调（p<0.05）。MM组与对照组之间MCP-1和IL-8的mRNA表达水平无显著差异（p>0.05）。结论：MM患者骨髓间充质干细胞表达HGF、SDF-1、MCP-1、IL-8，但MM患者骨髓微环境细胞成分中这些趋化相关因子的表达失调，这可能是MM细胞与间充质干细胞相互作用的结果。
