Zhang Yi-Zhuo, DA Wan-Ming
Department of Hematology, General Hospital of PLA, Beijing 100853, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2006 Apr;14(2):281-4.
This study was aimed to investigate the expression level of stromal cell derived factor-1 gene (SDF-1) in bone marrow mesenchymal stem cells (MSC) of patients with myelodysplastic syndrome (MDS). The MSC from bone marrow samples of MDS patients were isolated, cultured and expanded, the morphology and immunophenotype of MSC were analyzed. The expression levels of SDF-1 and internal reference GAPDH in MSC of MDS patients were detected by real-time quantitative reverse transcriptase polymerase chain reaction (RQ-RT-PCR) method and were compared with expression levels of healthy donors. The results showed that the expression levels of SDF-1 in MDS patients were significantly different from those in healthy donors (1.53 +/- 0.92 vs 5.51 +/- 0.99) (P < 0.01). SDF-1 gene expression levels in bone marrow MSC of MDS patients were significantly higher than that in MSC derived from healthy donors. It is concluded that the abnormal expression of SDF-1 gene in MSC may influence the regulation of hematopoiesis of the bone marrow microenvironment in MDS patients and it is worthy of further investigation for new clue on etiological mechanism and treatment of MDS.
本研究旨在探讨骨髓增生异常综合征（MDS）患者骨髓间充质干细胞（MSC）中基质细胞衍生因子-1基因（SDF-1）的表达水平。分离、培养并扩增MDS患者骨髓样本中的MSC，分析其形态和免疫表型。采用实时定量逆转录聚合酶链反应（RQ-RT-PCR）法检测MDS患者MSC中SDF-1和内参GAPDH的表达水平，并与健康供者的表达水平进行比较。结果显示，MDS患者SDF-1的表达水平与健康供者显著不同（1.53±0.92 vs 5.51±0.99）（P<0.01）。MDS患者骨髓MSC中SDF-1基因表达水平显著高于健康供者来源的MSC。结论：MSC中SDF-1基因的异常表达可能影响MDS患者骨髓微环境的造血调控，值得进一步研究以寻找MDS病因机制和治疗的新线索。
