Buchtová L, Láznicka M, Bilýk I, Hrkal Z
Fakulta vseobecného lékarství University Karlovy, Praha.
Vnitr Lek. 1990 Aug;36(8):774-8.
The authors tested three ways of freezing red cell concentrates with glycerin. At -30 degrees C in a deep freezer; on a Planer at the rate of 1 degree C per minute and by immersion into liquid nitrogen to -196 degrees C, stored in cryo-containers in liquid nitrogen. In samples processed in all three ways, incl. the follow-up of glycolysis in red cell concentrates in acid-citrate-dextrose solution, the levels of the following enzymes were assessed: glucose-6-phosphate dehydrogenase, glutathione reductase, pyruvate kinase. In addition also the levels of sodium, potassium, pH and glucose were assessed. The enzyme estimations of all three mentioned enzymes after triple rinsing with saline in the samples immersed in liquid nitrogen at -196 degrees C had the most acceptable values and smallest losses after rinsing.
作者测试了三种用甘油冷冻红细胞浓缩物的方法。在-30℃的低温冰箱中；在Planer上以每分钟1℃的速率进行冷冻；以及浸入液氮至-196℃，并储存在液氮中的低温容器中。在通过所有三种方式处理的样本中，包括在酸性枸橼酸盐葡萄糖溶液中对红细胞浓缩物糖酵解的后续研究，评估了以下酶的水平：葡萄糖-6-磷酸脱氢酶、谷胱甘肽还原酶、丙酮酸激酶。此外，还评估了钠、钾、pH值和葡萄糖的水平。在-196℃浸入液氮的样本中，用盐水三次冲洗后，上述三种酶的酶活性测定值最可接受，冲洗后的损失最小。
