Buchtová L, Láznicka M
Fakulta vseobecného lékarství Univerzity Karlovy, Praha.
Vnitr Lek. 1990 Aug;36(8):779-82.
The authors investigated changes of total lipids, cholesterol, triacyglycerols in red cell concentrates collected into acid-citrate-dextrose solution before freezing with glycerin and after freezing with glycerin to -30 degrees C in a deep freezer and to -196 degrees C by immersion into liquid nitrogen. It was revealed that the triacylglycerol levels after deglycerolization increased approximately three times. In transfusions of major amounts of thus processed blood the possibly impaired tissue saturation with oxygen will have to be taken into account. It may be concluded that lipids released from red cells impaired as a result of freezing may contribute to the formation of undesirable aggregates in blood.
作者研究了在使用甘油冷冻前和使用甘油冷冻至-30℃（在深冻箱中）以及通过浸入液氮冷冻至-196℃之前，收集到酸性枸橼酸盐葡萄糖溶液中的红细胞浓缩物中总脂质、胆固醇、甘油三酯的变化。结果显示，去甘油化后的甘油三酯水平大约增加了三倍。在大量输注如此处理过的血液时，必须考虑到可能受损的组织氧饱和度。可以得出结论，因冷冻而受损的红细胞释放出的脂质可能有助于血液中形成不良聚集体。
