O'Shea-Alvarez M S, Arguello-López C, González-Robles A, Treviño-García Manzo N
Hospital de Especialidades CMN-IMSS, México, D.F.
Arch Invest Med (Mex). 1990;21 Suppl 1:273-6.
The presence of IgA anti-Entamoeba histolytica antibodies has been demonstrates in intestinal secretions and serum of human amebiasis. We investigated which are the cellular components of trophozoites that react with IgA anti-ameba antibodies from immune serum, colostrum and human milk. The cellular localization of such antigens was accomplished by an indirect immunoperoxidase technique using anti-human IgA (alpha chain specific) labeled with peroxidase, both for light and transmission electron microscopy. Intracellular antigens were localized after permeating the parasites with cold acetone (-10 degrees C) for 3 min. and cryosections of 1 micron thick. The antigens that react with IgA antibodies from immune serum, colostrum and human milk were located in the plasma membrane and the internal portion of some cytoplasmic vesicles. So far, it is unknown what is the biological function of IgA in human amebiasis but in other systems it protects against certain parasites.
在人类阿米巴病患者的肠道分泌物和血清中已证实存在抗溶组织内阿米巴的IgA抗体。我们研究了滋养体中哪些细胞成分会与来自免疫血清、初乳和人乳的抗阿米巴IgA抗体发生反应。通过间接免疫过氧化物酶技术，使用过氧化物酶标记的抗人IgA（α链特异性）来完成此类抗原的细胞定位，用于光学显微镜和透射电子显微镜观察。在用冷丙酮（-10℃）处理寄生虫3分钟并制成1微米厚的冰冻切片后，对细胞内抗原进行定位。与免疫血清、初乳和人乳中的IgA抗体发生反应的抗原位于质膜和一些细胞质小泡内部。到目前为止，IgA在人类阿米巴病中的生物学功能尚不清楚，但在其他系统中它可抵御某些寄生虫。
