WestCHEM, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow G1 1XL, UK.
Chem Soc Rev. 2011 May;40(5):2279-92. doi: 10.1039/c0cs00150c. Epub 2011 Mar 1.
Hydrogenases catalyse redox reactions with molecular hydrogen, either as substrate or product. The enzymes harness hydrogen as a reductant using metals that are abundant and economical, namely, nickel and iron, and should provide new pointers for the economic use of hydrogen in manmade devices. The most recently discovered and perhaps the most enigmatic of the hydrogenases is the [Fe]-hydrogenase, used by certain microorganisms in the pathway that reduces carbon dioxide to methane. Since its discovery some twenty years ago, [Fe]-hydrogenase has consistently provided structural and mechanistic surprises, often requiring complete re-evaluation of its mechanism of action. This tutorial review combines recent advances in X-ray crystallography and other analytical techniques, as well as in computational studies and in chemical synthesis to provide a platform for understanding this remarkable enzyme type.
氢化酶催化与分子氢的氧化还原反应，既可以作为底物，也可以作为产物。这些酶利用丰富且经济的金属（镍和铁）来利用氢气作为还原剂，这为在人造设备中经济地使用氢气提供了新的思路。最近发现的、也许是最神秘的氢化酶是[Fe]-氢化酶，某些微生物在将二氧化碳还原为甲烷的途径中使用该酶。自二十年前发现以来，[Fe]-氢化酶一直不断地提供结构和机制上的惊喜，这常常需要对其作用机制进行全面重新评估。本综述结合了 X 射线晶体学和其他分析技术、计算研究以及化学合成方面的最新进展，为理解这种非凡的酶类型提供了一个平台。
