Mohammed Abdullahi, Abdul-Wahab Mohd Firdaus, Hashim Mazlan, Omar Abdul Hafidz, Md Reba Mohd Nadzri, Muhamad Said Mohd Farid, Soeed Kamaruzaman, Alias Siti Aisyah, Smykla Jerzy, Abba Mustapha, Ibrahim Zaharah
Ibrahim Badamasi Babangida University, Lapai, Niger State, Nigeria ; Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, Johor, Malaysia.
Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, Johor, Malaysia.
Pol J Microbiol. 2018;67(3):283-290. doi: 10.21307/pjm-2018-033.
Lower temperature biohydrogen production has always been attractive, due to the lower energy requirements. However, the slow metabolic rate of psychrotolerant biohydrogen-producing bacteria is a common problem that affects their biohydrogen yield. This study reports on the improved substrate synthesis and biohydrogen productivity by the psychrotolerant Klebsiella sp. strain ABZ11, isolated from Antarctic seawater sample. The isolate was screened for biohydrogen production at 30°C, under facultative anaerobic condition. The isolate is able to ferment glucose, fructose and sucrose with biohydrogen production rate and yield of 0.8 mol/l/h and 3.8 mol/g, respectively at 10 g/l glucose concentration. It also showed 74% carbohydrate uptake and 95% oxygen uptake ability, and a wide growth temperature range with optimum at 37°C. Klebsiella sp. ABZ11 has a short biohydrogen production lag phase, fast substrate uptake and is able to tolerate the presence of oxygen in the culture medium. Thus, the isolate has a potential to be used for lower temperature biohydrogen production process.
由于能量需求较低，低温生物制氢一直备受关注。然而，耐冷产氢细菌代谢速率缓慢是影响其生物氢产量的一个普遍问题。本研究报道了从南极海水样品中分离出的耐冷克雷伯氏菌属ABZ11菌株在底物合成和生物氢生产力方面的改善情况。该分离株在兼性厌氧条件下于30°C进行产氢筛选。该分离株能够发酵葡萄糖、果糖和蔗糖，在葡萄糖浓度为10 g/l时，产氢速率和产量分别为0.8 mol/l/h和3.8 mol/g。它还表现出74%的碳水化合物摄取能力和95%的氧气摄取能力，生长温度范围广，最适温度为37°C。克雷伯氏菌属ABZ11产氢的延迟期短，底物摄取快，并且能够耐受培养基中氧气的存在。因此，该分离株具有用于低温生物制氢过程的潜力。
