Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA.
Lab Chip. 2011 Apr 7;11(7):1276-9. doi: 10.1039/c0lc00684j. Epub 2011 Mar 1.
We report a portable lensless on-chip microscope that can achieve <1 µm resolution over a wide field-of-view of ∼ 24 mm(2) without the use of any mechanical scanning. This compact on-chip microscope weighs ∼ 95 g and is based on partially coherent digital in-line holography. Multiple fiber-optic waveguides are butt-coupled to light emitting diodes, which are controlled by a low-cost micro-controller to sequentially illuminate the sample. The resulting lensfree holograms are then captured by a digital sensor-array and are rapidly processed using a pixel super-resolution algorithm to generate much higher resolution holographic images (both phase and amplitude) of the objects. This wide-field and high-resolution on-chip microscope, being compact and light-weight, would be important for global health problems such as diagnosis of infectious diseases in remote locations. Toward this end, we validate the performance of this field-portable microscope by imaging human malaria parasites (Plasmodium falciparum) in thin blood smears. Our results constitute the first-time that a lensfree on-chip microscope has successfully imaged malaria parasites.
我们报告了一种便携式无透镜片上显微镜，它可以在不使用任何机械扫描的情况下实现 <1 µm 的分辨率，视场（FOV）约为 24mm(2)。这种紧凑型片上显微镜重约 95 克，基于部分相干数字共焦全息术。多个光纤波导与发光二极管进行背对背耦合，由低成本微控制器控制，以顺序照明样品。然后，由数字传感器阵列捕获无透镜全息图，并使用像素超分辨率算法快速处理，生成物体的更高分辨率全息图像（相位和幅度）。这种具有宽视场和高分辨率的片上显微镜，由于其紧凑轻便，对于全球健康问题（如偏远地区传染病的诊断）非常重要。为此，我们通过对薄血涂片进行成像，验证了这种现场便携式显微镜的性能，成功地对人体疟疾寄生虫（恶性疟原虫）进行了成像。我们的结果首次证明了无透镜片上显微镜可以成功地对疟疾寄生虫进行成像。
