College of Animal Science and Technology, Northwest A & F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, PR China.
Gen Comp Endocrinol. 2011 Jan 1;170(1):189-92. doi: 10.1016/j.ygcen.2010.10.004. Epub 2010 Oct 15.
Neuropeptide Y (NPY) is one of the most potent orexigenic factors that is implicated in the control of feeding behavior and energy homeostasis. Four Chinese indigenous cattle breeds (n=338) were used to detect single nucleotide polymorphisms (SNPs) in the coding regions with their intron-exon boundaries and the proximal flanking regions of NPY gene by PCR-SSCP and DNA sequencing. Five novel SNPs (38017 C>G, 34240 C>A, 34168 G>A, 32463 A>C and 32302 C>G) were identified. Polymorphisms of P3 lous in NPY gene, containing SNP4 and SNP5, were associated with body length and chest girth in Nanyang cattle aged 6, 12 and 18 months (P<0.05), but they had no significant effect on the two growth traits in Nanyang cattle aged 24 months (P>0.05). The results open a new field of study and extend the spectrum of genetic variation of the bovine NPY gene, which might contribute to cattle genetic resources and breeding.
神经肽 Y（NPY）是最有效的食欲因子之一，参与摄食行为和能量平衡的控制。本研究选用中国 4 个地方黄牛品种（n=338），应用 PCR-SSCP 和 DNA 测序技术，检测了 NPY 基因的编码区及其内含子-外显子边界、近端侧翼序列的单核苷酸多态性（SNPs）。共发现了 5 个新的 SNPs（38017 C>G、34240 C>A、34168 G>A、32463 A>C 和 32302 C>G）。NPY 基因 P3 区的 SNP4 和 SNP5 与南阳牛 6、12 和 18 月龄的体斜长和胸围显著相关（P<0.05），但对 24 月龄的这两个生长性状无显著影响（P>0.05）。该研究结果扩展了牛 NPY 基因遗传变异的范围，为牛遗传资源和选育开辟了新的研究领域。
