Singh M P, Pandey V K, Srivastava A K, Viswakarma S K
V.B.S. Purvanchal University, Department of Biotechnology, Jaunpur, India.
Cell Mol Biol (Noisy-le-grand). 2011 Feb 12;57(1):47-55.
The white rot fungus P. eryngii was grown on chemically and hot water treated agrowaste Brassica haulms. The fungus degraded lignin, cellulose, hemicellulose and carbon content of both chemically as well as hot water treated waste and produced in turn the edible and nutritious fruiting body. The progressive breakdown of lignin, cellulose and hemicellulose was correlated with apparent increase in the activities of lignolytic, cellulolytic and hemicellulolytic enzymes. Lignin degraded at faster rate during the vegetative phase and at slower rate during reproductive phase whereas, cellulose and hemicellulose depleted slowly during vegetative phase and rapidly during reproductive phase. The carbon content of the agrowaste decreased while, the nitrogen content increased and the C/N ratio came closer during degradation of the waste. Hot water treated substrate supported better production of enzymatic activity and degraded more efficiently than chemically sterilized substrate. The total yield and biological efficiency of the mushroom was the maximum on the hot water treated substrates.
白腐真菌杏鲍菇生长在经过化学处理和热水处理的农业废弃物芸苔秸秆上。该真菌降解了经化学处理和热水处理的废弃物中的木质素、纤维素、半纤维素和碳含量，进而产生了可食用且营养丰富的子实体。木质素、纤维素和半纤维素的逐步分解与木质素分解酶、纤维素分解酶和半纤维素分解酶活性的明显增加相关。木质素在营养生长阶段降解速度较快，在生殖阶段降解速度较慢，而纤维素和半纤维素在营养生长阶段消耗缓慢，在生殖阶段消耗迅速。在废弃物降解过程中，农业废弃物的碳含量降低，氮含量增加，碳氮比缩小。热水处理的底物比化学灭菌的底物更能支持酶活性的产生，且降解效率更高。在热水处理的底物上，蘑菇的总产量和生物学效率最高。
