Xie Chunliang, Luo Wei, Li Zhimin, Yan Li, Zhu Zuohua, Wang Jing, Hu Zhenxiu, Peng Yuande
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, P. R. China.
Electrophoresis. 2016 Jan;37(2):310-20. doi: 10.1002/elps.201500312. Epub 2015 Nov 27.
Pleurotus eryngii (P. eryngii) can secrete large amount of hydrolytic and oxidative enzymes to degrade lignocellulosic biomass. In spite of several researches on the individual lignolytic enzymes, a direct deconstruction of lignocellulose by enzyme mixture is not yet possible. Identifying more high-performance enzymes or enzyme complexes will lead to efficient in vitro lignocelluloses degradation. In this report, secretomic analysis was used to search for the new or interesting enzymes for lignocellulose degradation. Besides, the utilization ability of P. eryngii to ramie stalk substrate was evaluated from the degradation of cellulose, hemicellulose, and lignin in medium and six extracellular enzymes activities during different growth stages were discussed. The results showed that a high biological efficiency of 71% was obtained; cellulose, hemicelluloses, and lignin decomposition rates of P. eryngii were 29.2, 26.0, and 51.2%, respectively. Enzyme activity showed that carboxymethyl cellulase, xylanase, laccase, and peroxidase activity peaks appeared at the primordial initiation stage. In addition, we profiled a global view of the secretome of P. eryngii cultivated in ramie stalk media to understand the mechanism behind lignocellulosic biomass hydrolysis. Eighty-seven nonredundant proteins were identified and a diverse group of enzymes, including cellulases, hemicellulases, pectinase, ligninase, protease, peptidases, and phosphatase implicated in lignocellulose degradation were found. In conclusion, the information in this report will be helpful to better understand the lignocelluloses degradation mechanisms of P. eryngii.
杏鲍菇能够分泌大量水解酶和氧化酶来降解木质纤维素生物质。尽管对单个木质素分解酶已有多项研究，但利用酶混合物直接解构木质纤维素仍不可行。鉴定出更多高性能的酶或酶复合物将有助于实现高效的体外木质纤维素降解。在本报告中，采用分泌蛋白质组学分析来寻找用于降解木质纤维素的新酶或有趣的酶。此外，从培养基中纤维素、半纤维素和木质素的降解情况评估了杏鲍菇对苎麻茎秆底物的利用能力，并讨论了不同生长阶段六种细胞外酶的活性。结果表明，获得了71%的高生物效率；杏鲍菇对纤维素、半纤维素和木质素的分解率分别为29.2%、26.0%和51.2%。酶活性显示，羧甲基纤维素酶、木聚糖酶、漆酶和过氧化物酶的活性峰值出现在原基起始阶段。此外，我们对在苎麻茎秆培养基中培养的杏鲍菇分泌蛋白质组进行了全面分析，以了解木质纤维素生物质水解背后的机制。鉴定出了87种非冗余蛋白质，并发现了一组不同的酶，包括参与木质纤维素降解的纤维素酶、半纤维素酶、果胶酶、木质素酶、蛋白酶、肽酶和磷酸酶。总之，本报告中的信息将有助于更好地理解杏鲍菇的木质纤维素降解机制。
