Ayala A, Perrin M M, Wagner M A, Chaudry I H
Department of Surgery, Michigan State University, East Lansing 48824-1315.
Arch Surg. 1990 Jan;125(1):70-4; discussion 74-5. doi: 10.1001/archsurg.1990.01410130076010.
To determine whether phagocytosis mediated by Fc receptors and/or receptors for the third component of complement (C3b) are altered after hemorrhage, C3H/HeN mice were subjected to nonlethal hemorrhage and then adequately resuscitated. Twelve hours after the hemorrhagic episode, a significant decrease in both Fc (-55.2%) and C3b (-46.6%) receptor-positive peritoneal macrophages was observed compared with controls. At 24 hours the extent of the depression, while still marked, was only -22.5% and -17.4% for Fc and C3b receptors, respectively. By day 3 after hemorrhage, no differences could be observed for either of these receptors. The capacity of macrophages from mice after hemorrhage to elaborate interleukin 1 or tumor necrosis factor-alpha showed no increase over that of the sham controls, and serum levels of endotoxin were not elevated 2 or 24 hours after hemorrhage. Moreover, endotoxin-tolerant C3H/HeJ mice also exhibited depression of both receptors after hemorrhage. Thus, the inability of the host macrophages to clear opsonized infectious agents after hemorrhage may be due in part to the loss of Fc and C3b receptors on macrophages.
为了确定出血后由Fc受体和/或补体第三成分（C3b）受体介导的吞噬作用是否发生改变，对C3H/HeN小鼠进行非致死性出血，然后进行充分复苏。出血事件发生12小时后，与对照组相比，Fc受体（-55.2%）和C3b受体（-46.6%）阳性的腹腔巨噬细胞均显著减少。在24小时时，Fc和C3b受体的抑制程度虽然仍然显著，但分别仅为-22.5%和-17.4%。出血后第3天，这两种受体均未观察到差异。出血后小鼠巨噬细胞分泌白细胞介素1或肿瘤坏死因子-α的能力与假手术对照组相比没有增加，出血后2小时或24小时血清内毒素水平也没有升高。此外，对内毒素耐受的C3H/HeJ小鼠出血后也表现出两种受体的抑制。因此，宿主巨噬细胞在出血后无法清除调理素化的感染因子可能部分归因于巨噬细胞上Fc和C3b受体的丧失。
