Vĕtvicka V, Fornůsek L, Zídková J
Immunology. 1985 Sep;56(1):73-80.
Age-dependent changes in the expression of Fc receptors (FcR) for different isotypes of immunoglobulins and receptors for C3b, C5b and C3bi fragments of complement on the membranes of peritoneal macrophages were studied with mice of different ages. An age-related increase in expression of Fc receptors for IgM, IgE, IgA, IgG2b and IgG3, and a decrease in the expression of Fc receptors for IgG1 was observed. The expression of FcR on macrophages of donors of different ages corresponded with Fc-receptor mediated phagocytosis. The highest number of C3b-binding macrophages was found in aged mice, in contrast to low numbers of C3bi-binding macrophages at this age. The percentage of C5b-binding macrophages was lowest in adult animals. We also observed effective inhibition of binding of the C3b component of complement by preincubation of macrophages with aggregated IgG and vice versa. These observations suggest that fluctuation in expression of Fc but not C receptors may be important to the generalized changes that occur in macrophage function during development and ageing.
利用不同年龄的小鼠，研究了腹膜巨噬细胞膜上针对不同免疫球蛋白同种型的Fc受体（FcR）以及补体C3b、C5b和C3bi片段受体的表达随年龄的变化。观察到IgM、IgE、IgA、IgG2b和IgG3的Fc受体表达随年龄增加，而IgG1的Fc受体表达降低。不同年龄供体巨噬细胞上FcR的表达与Fc受体介导的吞噬作用相对应。在老年小鼠中发现结合C3b的巨噬细胞数量最多，与此形成对比的是，这个年龄段结合C3bi的巨噬细胞数量较少。结合C5b的巨噬细胞百分比在成年动物中最低。我们还观察到，巨噬细胞与聚集的IgG预孵育可有效抑制补体C3b成分的结合，反之亦然。这些观察结果表明，Fc而非C受体表达的波动可能对发育和衰老过程中巨噬细胞功能发生的普遍变化具有重要意义。
