Department for Plastic Surgery and Hand Surgery, Klinikum rechts der Isar, Technische Universität München, Ismaninger Str. 22, 81675, Munich, Germany.
Langenbecks Arch Surg. 2011 Apr;396(4):543-50. doi: 10.1007/s00423-011-0761-3. Epub 2011 Mar 3.
With the development of cell-based gene transfer techniques, genetically modified human keratinocytes (Kc) and fibroblasts (Fb) have been proven to be a better choice in wound repair.
This study was designed to construct in one step a gene-modified artificial skin by a genetically engineered Kc expressing PDGF-BB and Fb expressing VEGF(165) and bFGF. The wound healing effect in a full-thickness wound model was then observed. Unmodified artificial skin served as control. On the post-operative days 7, 14, and 21, residual wound area was calculated and skin wound tissues were subjected to biopsy for further investigation.
Compared with unmodified artificial skin, gene-modified artificial skin resulted in a reduced wound contraction and a well-organized human epidermis and better formed dermis.
The results suggest that our two-layer, gene-modified artificial skin improved both vascularization and epidermalization for skin regeneration. This technique could bring about a new approach in the treatment of burns and chronic wounds.
随着基于细胞的基因转移技术的发展，已证明基因修饰的人角质形成细胞（Kc）和成纤维细胞（Fb）在伤口修复中是更好的选择。
本研究旨在通过表达 PDGF-BB 的基因修饰 Kc 和表达 VEGF(165)和 bFGF 的 Fb 一步构建基因修饰的人工皮肤，然后观察其在全层创面模型中的愈合效果。未修饰的人工皮肤作为对照。在术后第 7、14 和 21 天，计算残余创面面积，并对皮肤创面组织进行活检以进一步研究。
与未修饰的人工皮肤相比，基因修饰的人工皮肤可减少创面收缩，形成组织有序的人表皮和更好形成的真皮。
结果表明，我们的两层基因修饰人工皮肤改善了皮肤再生的血管化和表皮化。该技术可为烧伤和慢性创面的治疗带来新方法。
