Hernon Catherine A, Dawson Rebecca A, Freedlander Eric, Short Robert, Haddow David B, Brotherston Michael, MacNeil Sheila
Tissue Engineering Group, Department of Engineering Materials, Kroto Research Institute, University of Sheffield North Campus, Sheffield, UK.
Regen Med. 2006 Nov;1(6):809-21. doi: 10.2217/17460751.1.6.809.
We report a 10-year audit using cultured epithelial autografts (CEAs) for patients with extensive burns. Clinical take using CEAs averaged only 45% (as has been reported by others) but over half of all cells cultured for these patients had to be discarded owing to difficulties of timing the production of CEA sheets to the needs of the patients. CEAs could not be used until they had reached confluence and formed an integrated sheet, which took, on average, 12 days. However, once formed, they needed to be used within 2-3 days or they lost the ability to attach to wound beds. In response to this we developed a simpler carrier dressing methodology for transferring cultured subconfluent keratinocytes from the laboratory to the wound bed. This methodology offers an increase in speed of delivery but its major contribution is the greater flexibility in timing the transfer of cells from the laboratory to the changing needs of the patients.
我们报告了一项针对大面积烧伤患者使用培养上皮自体移植（CEA）的为期10年的审计。使用CEA的临床成功率平均仅为45%（如其他人所报告），但由于CEA片的生产时间难以与患者需求相匹配，这些患者培养的所有细胞中有超过一半不得不被丢弃。CEA在达到汇合并形成完整薄片之前不能使用，平均需要12天。然而，一旦形成，它们需要在2 - 3天内使用，否则就会失去附着在创面床的能力。针对这一情况，我们开发了一种更简单的载体敷料方法，用于将培养的未汇合角质形成细胞从实验室转移到创面床。这种方法提高了输送速度，但其主要贡献在于在根据患者不断变化的需求安排细胞从实验室转移的时间方面具有更大的灵活性。
