Burgemeister Renate
Carl Zeiss MicroImaging, München, Germany.
Methods Mol Biol. 2011;724:105-15. doi: 10.1007/978-1-61779-055-3_7.
Laser capture microdissection (LCM) enables researchers to combine structure identification by -microscopy with structure investigation by modern molecular techniques.The main question in modern biomedical research is the understanding of cellular and molecular mechanisms. The methods to investigate pathological changes on a molecular, cellular, or tissue level become more and more exact, whereas at the same time the sample amounts available become smaller and smaller.The challenge in microscopy is the identification of structures or molecules. Today, scientists are no longer satisfied with just observing tissues and cells. They demand the ability to get access to the identified structures to bring their observations to the subcellular and genetic level. Downstream to microscopy the full toolbox of molecular biology for DNA, RNA, and protein analysis has to be applied.
激光捕获显微切割（LCM）使研究人员能够将显微镜下的结构识别与现代分子技术的结构研究相结合。现代生物医学研究中的主要问题是对细胞和分子机制的理解。在分子、细胞或组织水平上研究病理变化的方法越来越精确，而与此同时，可用的样本量却越来越小。显微镜技术面临的挑战是识别结构或分子。如今，科学家们不再满足于仅仅观察组织和细胞。他们要求能够获取已识别的结构，以便将观察深入到亚细胞和基因水平。在显微镜技术之后，必须应用用于DNA、RNA和蛋白质分析的全套分子生物学工具。
