Interdepartmental Center of Tissue Engineering, University of Pavia, Italy.
J Biomed Mater Res A. 2011 May;97(2):118-26. doi: 10.1002/jbm.a.32996. Epub 2011 Mar 2.
Three-dimensional (3D) titanium-6-aluminium-4-vanadium (Ti6Al4V) is a widely used biomaterial for orthopedic prosthesis and dental implants; thanks to its very high-mechanical strength and resistance to corrosion. Human mesenchymal stem cells (hMSCs) and dental pulp stem cells (hDPSCs) are responsible for bone regeneration following colonization of prosthesis or dental implants. Both hMSCs and hDPSCs have lower ability to colonize this biomaterial in comparison with tissue culture-treated plastic. Both hMSCs and hDPSCs show lack of focal adhesion kinase (FAK) activation when grown on Ti6Al4V. This signal is restored in the presence of poly-L-lysine (poly-L-lys). Poly-L-lys has been used as part of organoapatite or together with zinc and calcium ions. Our results suggest that poly-L-lys alone induces FAK activation through β1-INTEGRIN, because the presence of β1-INTEGRIN blocking antibody avoided FAK autophosphorylation. Presence of poly-L-lys also increases expression of osteoblastic differentiation marker genes in hMSCs and hDPSCs grown on Ti6Al4V.
三维（3D）钛-6-铝-4-钒（Ti6Al4V）是一种广泛用于骨科假体和牙科植入物的生物材料；由于其非常高的机械强度和耐腐蚀性。人骨髓间充质干细胞（hMSCs）和牙髓干细胞（hDPSCs）负责在假体或牙科植入物定植后进行骨再生。与组织培养处理的塑料相比，hMSCs 和 hDPSCs 定植这种生物材料的能力较低。当在 Ti6Al4V 上生长时，hMSCs 和 hDPSCs 均显示出缺乏粘着斑激酶（FAK）的激活。在聚-L-赖氨酸（poly-L-lys）的存在下，这种信号得以恢复。聚-L-赖氨酸已被用作有机磷灰石的一部分或与锌和钙离子一起使用。我们的结果表明，聚-L-赖氨酸通过β1-整合素单独诱导 FAK 激活，因为β1-整合素阻断抗体的存在阻止了 FAK 的自身磷酸化。聚-L-赖氨酸的存在还增加了在 Ti6Al4V 上生长的 hMSCs 和 hDPSCs 中成骨细胞分化标记基因的表达。
