Hussain Ibrahim, Moharamzadeh Keyvan, Brook Ian M, José de Oliveira Neto Patrício, Salata Luiz A
School of Clinical Dentistry, The University of Sheffield, Claremont Crescent, Sheffield S10 2TA, UK.
Int J Dent. 2012;2012:396316. doi: 10.1155/2012/396316. Epub 2012 Mar 15.
The aim of this study was to assess the osteoconductive and osteogenic properties of processed bovine dentin using a robust rabbit calvarial defect model. In total, 16 New Zealand White rabbits were operated to create three circular defects in the calvaria. One defect was left unfilled, one filled with collected autogenous bone, and the third defect was filled with the dentin-based bone substitute. Following surgery and after a healing period of either 1 or 6 weeks, a CT scan was obtained. Following sacrificing, the tissues were processed for histological examination. The CT data showed the density in the area grafted with the dentin-based material was higher than the surrounding bone and the areas grafted with autologous bone after 1 week and 6 weeks of healing. The area left unfilled remained an empty defect after 1 week and 6 weeks. Histological examination of the defects filled with the dentin product after 6 weeks showed soft tissue encapsulation around the dentin particles. It can be concluded that the rabbit calvarial model used in this study is a robust model for the assessment of bone materials. Bovine dentin is a biostable material; however, it may not be suitable for repairing large 4-wall defects.
本研究的目的是使用可靠的兔颅骨缺损模型评估处理过的牛牙本质的骨传导性和成骨特性。总共对16只新西兰白兔进行手术，在颅骨上制造三个圆形缺损。一个缺损不填充，一个填充收集的自体骨，第三个缺损填充牙本质基骨替代物。手术后以及1周或6周的愈合期后，进行CT扫描。处死动物后，对组织进行处理以进行组织学检查。CT数据显示，在愈合1周和6周后，移植牙本质基材料的区域密度高于周围骨以及移植自体骨的区域。未填充的区域在1周和6周后仍为空洞缺损。6周后对填充牙本质产品的缺损进行组织学检查，结果显示牙本质颗粒周围有软组织包裹。可以得出结论，本研究中使用的兔颅骨模型是评估骨材料的可靠模型。牛牙本质是一种生物稳定性材料；然而，它可能不适合修复大型四壁缺损。
