Department of Anatomy and Cell Biology, National Yang-Ming University, Taipei, Taiwan.
Histopathology. 2011 Mar;58(4):593-600. doi: 10.1111/j.1365-2559.2011.03783.x. Epub 2011 Mar 3.
The severity of cartilage degeneration is positively correlated with the severity of the pathologic change of medial plica. However, knowledge of the pathogenic mechanisms and the impact of plica on cartilage destruction is limited. The aim of the present study was therefore to investigate matrix metalloprotease-3 (MMP-3) expression in the plica isolated from patients with medial compartment osteoarthritis of the knee.
Immunohistochemistry showed that MMP-3 was highly expressed in pannus-like tissue and the plica. Western blotting of culture supernatants showed that interleukin-1β (IL-1β) treatment induced MMP-3 release by cells isolated from pannus tissue or the plica. Furthermore, reverse transcriptase polymerase chain reaction and real-time polymerase chain reaction analysis showed that MMP-3 mRNA levels were increased after IL-1β treatment of the cultured cells. MMP-3 and IL-1β mRNAs were expressed in the plica and pannus-like tissue, with MMP-3 mRNA being expressed at significantly higher levels in the plica than in normal synovial membrane and highly expressed in the plica at different stages in osteoarthritis (OA) patients.
Pannus-like tissue and the plica express IL-1β and MMP-3. Moreover, MMP-3 mRNA and protein expression in the plica may contribute to the pathogenesis of OA.
软骨退变的严重程度与内侧皱襞病理变化的严重程度呈正相关。然而，关于皱襞的致病机制及其对软骨破坏的影响知之甚少。因此，本研究旨在探讨膝关节内侧间室骨关节炎患者皱襞中基质金属蛋白酶-3（MMP-3）的表达。
免疫组织化学显示 MMP-3 在滑膜肉芽组织和皱襞中高度表达。培养上清液的 Western blot 显示白细胞介素-1β（IL-1β）处理诱导来自滑膜肉芽组织或皱襞的细胞释放 MMP-3。此外，逆转录聚合酶链反应和实时聚合酶链反应分析显示，培养细胞经 IL-1β 处理后 MMP-3 mRNA 水平增加。MMP-3 和 IL-1β mRNA 在皱襞和滑膜肉芽组织中表达，MMP-3 mRNA 在皱襞中的表达水平明显高于正常滑膜，在骨关节炎（OA）患者的不同阶段均高度表达。
滑膜肉芽组织和皱襞表达 IL-1β 和 MMP-3。此外，皱襞中 MMP-3 mRNA 和蛋白的表达可能有助于 OA 的发病机制。
