National Institute of Virology, Microbial Containment Complex, Pune, India.
Trop Med Int Health. 2011 May;16(5):585-8. doi: 10.1111/j.1365-3156.2011.02743.x. Epub 2011 Mar 3.
To identify the aetiological agent/s of an outbreak of chikungunya-like illness with high morbidity and several fatalities in Tamil Nadu, India, 2009-2010.
Two hundred and seventeen serum samples were collected from the affected areas and screened for chikungunya virus (CHIKV), dengue virus (DENV) and Japanese encephalitis virus (JEV) IgM antibodies using MAC-ELISA kits. A few selected samples were also tested for Ross River, Sindbis, and Murrey Valley viruses by RT-PCR and Hantan virus by serology. Twelve acute serum and mosquito samples were processed for virus isolation in C6/36 cells. CHIKV isolate was characterised by RT-PCR and sequencing.
Diagnostic levels of IgM antibodies were detected in 107 (49.3%) CHIKV samples and 22 (10.1%) DENV samples. IgM antibodies against JEV were not detected (n=46). Characterisation of the CHIKV isolate at genetic level demonstrated it as ECSA (E1: 226A). Thirty-six selected samples were also negative for Ross River, Sindbis, Murrey Valley and Hantan viruses.
High prevalence of CHIKV IgM antibody positivity, clinical symptoms, virus isolation and the presence of vector mosquitoes clearly suggest CHIKV as the aetiological agent responsible for the outbreak.
鉴定印度泰米尔纳德邦 2009-2010 年一起发病率高且有多人死亡的基孔肯雅样疾病暴发的病因。
从受影响地区采集了 217 份血清样本，并用 MAC-ELISA 试剂盒检测基孔肯雅病毒（CHIKV）、登革热病毒（DENV）和日本脑炎病毒（JEV）IgM 抗体。还通过 RT-PCR 对一些选定的样本检测了罗斯河病毒、辛德毕斯病毒和默里谷病毒，并用血清学方法检测了汉坦病毒。对 12 份急性血清和蚊子样本进行了病毒分离，在 C6/36 细胞中进行。通过 RT-PCR 和测序对 CHIKV 分离株进行了特征描述。
在 107 份（49.3%）CHIKV 样本和 22 份（10.1%）DENV 样本中检测到诊断水平的 IgM 抗体。未检测到 JEV 的 IgM 抗体（n=46）。CHIKV 分离株的基因特征表明其为 ECSA（E1：226A）。36 份选定样本也对罗斯河病毒、辛德毕斯病毒、默里谷病毒和汉坦病毒呈阴性。
高比例的 CHIKV IgM 抗体阳性率、临床症状、病毒分离和病媒蚊子的存在，明确表明 CHIKV 是导致此次暴发的病原体。
