Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães/Fiocruz, Recife, Brazil.
Hum Immunol. 2011 May;72(5):402-5. doi: 10.1016/j.humimm.2011.02.015. Epub 2011 Mar 1.
Previous works of our research group have demonstrated aspects of the humoral immune response of chronic Chagas disease using the cytoplasmatic repetitive antigen (CRA) and the flagellar repetitive antigen (FRA) of Trypanosoma cruzi. The aim of this work was to analyze the presence of specific immunoglobulin M (IgM) antibodies in chronic chagasic patients using these recombinant antigens of T. cruzi. The positivity of IgM in chronic chagasic patients against CRA and FRA antigens was determined by indirect enzyme-linked immunosorbent assay. We reported no statistical significant differences between the levels of IgM for both recombinant antigens and the different chronic clinical forms of Chagas disease. However, a small proportion of chronic chagasic patients analyzed in this study was positive for this antibody isotype. The findings of this study indicate that the IgM antibodies cannot be used to elucidate the differences in the profile of humoral immune response among chronic chagasic patients with different clinical forms using the CRA and FRA recombinant antigens of T. cruzi.
本研究小组之前的工作已经使用克氏锥虫的细胞质重复抗原（CRA）和鞭毛重复抗原（FRA）研究了慢性恰加斯病的体液免疫反应的各个方面。本工作旨在使用这些克氏锥虫重组抗原分析慢性恰加斯病患者是否存在特异性免疫球蛋白 M（IgM）抗体。通过间接酶联免疫吸附试验确定慢性恰加斯病患者针对 CRA 和 FRA 抗原的 IgM 阳性。我们报告说，两种重组抗原和不同慢性恰加斯病临床形式之间的 IgM 水平没有统计学显著差异。然而，在这项研究中分析的一小部分慢性恰加斯病患者的这种抗体同种型呈阳性。本研究的结果表明，IgM 抗体不能用于阐明使用 T. cruzi 的 CRA 和 FRA 重组抗原在不同临床形式的慢性恰加斯病患者之间体液免疫反应特征的差异。
