Verçosa A F A, Lorena V M B, Carvalho C L, Melo M F A D, Cavalcanti M G A, Silva E D, Ferreira A G P, Pereira V R A, Souza W V, Gomes Y M
Centro de Pesquisas Aggeu Magalhães (CPqAM)/Fiocruz, Recife-PE, Brazil.
J Clin Lab Anal. 2007;21(5):271-6. doi: 10.1002/jcla.20186.
The wide range of clinical Chagas' disease manifestations, of which heart involvement is the most significant, because of its characteristics, frequency and consequences, and lack of treatment and cure, justify research in this area. Specific immunoglobulin G (IgG) antibody subclasses have been associated with human Chagas' disease. Thus, in this study, the profile of IgG subclasses against cytoplasmic (CRA) and flagellar (FRA) recombinant repetitive T. cruzi-specific antigens was correlated with cardiac (CARD, n=33), cardiodigestive (CD, n=7), and indeterminate (IND, n=20) forms of Chagas' disease by indirect enzyme-linked immunosorbent assay (ELISA). IgG subclasses were detected in almost all Chagas patients studied. Nevertheless, only specific IgG2 isotype FRA was found with a significant statistical difference in CARD patients when compared to IND patients. This result suggests the potential use of this isotype for prognostic purposes, for monitoring the progression of chronic Chagas' disease, and for predicting the risk of CARD damage. This is important information, as it could help physicians to evaluate and manage the treatment of their patients. However, a follow-up study is necessary to confirm our result.
恰加斯病临床表现多样，其中心脏受累最为显著，因其特征、发生频率及后果，且缺乏有效治疗和治愈方法，故该领域的研究具有合理性。特定免疫球蛋白G（IgG）抗体亚类与人类恰加斯病相关。因此，在本研究中，通过间接酶联免疫吸附测定（ELISA），检测针对克氏锥虫细胞质重组重复抗原（CRA）和鞭毛重组重复抗原（FRA）的IgG亚类谱，并将其与恰加斯病的心脏型（CARD，n = 33）、心脏消化道型（CD，n = 7）和隐匿型（IND，n = 2）进行相关性分析。几乎在所有研究的恰加斯病患者中都检测到了IgG亚类。然而，与隐匿型患者相比，仅在心脏型患者中发现特异性IgG2同种型FRA存在显著统计学差异。该结果表明该同种型在预后评估、监测慢性恰加斯病进展以及预测心脏损伤风险方面具有潜在应用价值。这是重要信息，因为它有助于医生评估和管理患者的治疗。然而，需要进行后续研究以证实我们的结果。
