Division of Phytotherapeutics and Metabolic Endocrinology, Faculty of Science, The MS University of Baroda, Vadodara 390002, Gujarat, India.
Food Chem Toxicol. 2011 Jun;49(6):1195-202. doi: 10.1016/j.fct.2011.02.017. Epub 2011 Mar 1.
This study reports the protective role of Clerodendron glandulosum (CG) extract against in vitro LDL oxidation and Ox-LDL induced macrophage apoptosis using various experimental models. Effect of CG extract on Cu(2+) mediated LDL oxidation kinetics and formation of various intermediary products and its ability to prevent human monocyte derived macrophage mediated LDL oxidation have been investigated. Ox-LDL induced macrophage apoptosis was evaluated by nuclear condensation, cell cycle analysis, and annexin V-FITC/PI staining in presence or absence of CG extract. Results recorded in the present study clearly suggest the protective role of CG extract against LDL oxidation and Ox-LDL induced macrophage oxidative stress, mitochondrial dysfunction, and apoptosis. This is the first report on the protective role of CG extract on two key events of atherosclerosis portending its possible therapeutic use as an anti-atherogenic herbal medicine.
本研究报告了罗勒提取物（CG）对体外 LDL 氧化和 Ox-LDL 诱导的巨噬细胞凋亡的保护作用，使用了各种实验模型。研究了 CG 提取物对 Cu(2+)介导的 LDL 氧化动力学以及各种中间产物形成的影响，以及其预防人单核细胞源性巨噬细胞介导的 LDL 氧化的能力。通过核浓缩、细胞周期分析和在存在或不存在 CG 提取物的情况下用 Annexin V-FITC/PI 染色评估 Ox-LDL 诱导的巨噬细胞凋亡。本研究记录的结果清楚地表明 CG 提取物对 LDL 氧化和 Ox-LDL 诱导的巨噬细胞氧化应激、线粒体功能障碍和凋亡具有保护作用。这是首次报道 CG 提取物对动脉粥样硬化两个关键事件的保护作用，预示着它可能作为一种抗动脉粥样硬化的草药在治疗上的应用。
