Division of Phytotherapeutics and Metabolic Endocrinology, Department of Zoology, Faculty of Science, The M. S. University of Baroda, Vadodara, Gujarat, India.
Immunopharmacol Immunotoxicol. 2012 Jun;34(3):443-53. doi: 10.3109/08923973.2011.618136. Epub 2011 Sep 30.
Present inventory evaluates the anti-atherogenic potential of C. glandulosum.Coleb leaf extract (CG) using in vivo and in vitro experimental models. Serum markers of low density lipoprotein (LDL-C) oxidation, cholesterol, triglycerides, lipoproteins, auto-antibody titer, ex vivo LDL-C oxidation, LDL-C aggregation, aortic lipids, histopathological evaluations and immunolocalization of macrophage surface marker (F4/80), vascular cell adhesion molecule-1 (VCAM-1) and P-selectin were performed in CON [rats treated with single dose of saline (i.p.) and fed with laboratory chow], ATH [rats treated with single dose of vitamin D3 (600,000 IU, i.p) and fed with atherogenic diet] and ATH+CG [rats treated with single dose of vitamin D3 (600,000 IU, i.p.) and fed with atherogenic diet and simultaneously treated with 200 mg/kg CG extract, p.o.] for 8 weeks. CG extract supplementation to atherogenic diet fed rats significantly prevented increment in serum cholesterol, triglycerides, and lipoproteins, markers of LDL-C oxidation, auto-antibody titer and aortic lipids. Also, LDL-C isolated from ATH+CG rats recorded mimimal aggregation and susceptibility to undergo ex vivo LDL-C oxidation. Microscopic evaluation of thoracic aorta of ATH+CG rats reveled prevention of atheromatous plaque formation, accumulation of lipid laden macrophages, calcium deposition, distortion/defragmentation of elastin, accumulation of macrophages and, down regulation of cell adhesion molecules (VCAM-1 and P-selectin) expression. Further, in vitro monocyte to macrophage differentiation was significantly attenuated in presence of CG extract (200 µg/mL). It can be concluded from the present study that, CG extract is capable of controlling induction of experimental atherosclerosis and warrants further scrutiny at the clinical level as a possible therapeutic agent.
本研究旨在评估 C. glandulosum.Coleb 叶提取物（CG）的抗动脉粥样硬化作用。通过体内和体外实验模型对血清低密度脂蛋白（LDL-C）氧化标志物、胆固醇、甘油三酯、脂蛋白、自身抗体滴度、LDL-C 氧化、LDL-C 聚集、主动脉脂质、组织病理学评估和巨噬细胞表面标志物（F4/80）、血管细胞黏附分子-1（VCAM-1）和 P 选择素的免疫定位进行检测。CON [大鼠单次腹腔注射生理盐水（i.p.）并给予实验室饲料]、ATH [大鼠单次腹腔注射维生素 D3（600,000 IU，i.p.）并给予动脉粥样硬化饮食]和 ATH+CG [大鼠单次腹腔注射维生素 D3（600,000 IU，i.p.）并给予动脉粥样硬化饮食和同时给予 200 mg/kg CG 提取物，p.o.] 喂养 8 周。CG 提取物补充到动脉粥样硬化饮食喂养的大鼠中可显著防止血清胆固醇、甘油三酯和脂蛋白、LDL-C 氧化标志物、自身抗体滴度和主动脉脂质的增加。此外，ATH+CG 大鼠分离的 LDL-C 记录到最小的聚集和对体外 LDL-C 氧化的易感性。ATH+CG 大鼠胸主动脉的显微镜评估显示动脉粥样斑块形成的预防、脂质负荷巨噬细胞的积累、钙沉积、弹性蛋白的变形/碎片化、巨噬细胞的积累和细胞黏附分子（VCAM-1 和 P-选择素）表达的下调。此外，在 CG 提取物（200 µg/mL）存在的情况下，单核细胞向巨噬细胞分化明显减弱。从本研究可以得出结论，CG 提取物能够控制实验性动脉粥样硬化的诱导，并在临床水平上进一步研究作为一种潜在的治疗药物。
