Yuan J, Handy R D, Moody A J, Smerdon G, Bryson P
School of Biomedical and Biological Sciences, University of Plymouth, Drake Circus, Plymouth PL4 8AA, UK.
Biochim Biophys Acta. 2011 May;1810(5):526-31. doi: 10.1016/j.bbagen.2011.02.007. Epub 2011 Mar 1.
In vitro studies on hyperbaric oxygen (HBO) therapy suggest that HBO may cause DNA damage, but this has not been evaluated using endothelial cells.
Human umbilical cord endothelial cells (HUVECs) were exposed either to H(2)O(2) or to HBO for 90 min, with or without subsequent H(2)O(2) exposure. Measurements included the comet assay for DNA damage, and reduced and oxidised glutathione levels.
HUVECs showed sensitivity to H(2)O(2) (EC(50) of 0.2mM for DNA migration). A single 90 min HBO treatment at 2.2 ATA caused a statistically significant (ANOVA, P<0.05) increase of DNA migration in HUVECs to 6.8 ± 0.3% (mean ± SEM, n=8), which returned to normal levels (4.9 ± 0.1%, n=6) after 24h. Further exposure to 0.2mM H(2)O(2) after HBO treatment significantly increased the DNA migration in HBO-treated cells immediately post-treatment; but 24h later the cells showed 22% less DNA damage and higher glutathione than controls.
A single HBO exposure causes limited DNA damage to HUVECs, which repairs quickly. HBO treatment protects against H(2)O(2)-induced DNA damage and involves cellular glutathione.
Endothelial cells are unlikely to be compromised during HBO therapy.
对高压氧（HBO）疗法的体外研究表明，HBO可能会导致DNA损伤，但尚未使用内皮细胞对此进行评估。
将人脐静脉内皮细胞（HUVECs）暴露于过氧化氢（H₂O₂）或HBO中90分钟，有无后续H₂O₂暴露。测量包括用于DNA损伤的彗星试验以及还原型和氧化型谷胱甘肽水平。
HUVECs对H₂O₂敏感（DNA迁移的半数有效浓度（EC₅₀）为0.2mM）。在2.2ATA下进行单次90分钟的HBO处理导致HUVECs中的DNA迁移在统计学上显著增加（方差分析，P<0.05），达到6.8±0.3%（平均值±标准误，n = 8），24小时后恢复到正常水平（4.9±0.1%，n = 6）。HBO处理后进一步暴露于0.2mM H₂O₂会在处理后立即显著增加HBO处理细胞中的DNA迁移；但24小时后，这些细胞的DNA损伤比对照组少22%，且谷胱甘肽水平更高。
单次HBO暴露对HUVECs造成的DNA损伤有限，且修复迅速。HBO处理可防止H₂O₂诱导的DNA损伤，并涉及细胞谷胱甘肽。
在内皮细胞中，HBO疗法不太可能造成损害。
