Institute of Chemistry, Department of Analytical Chemistry, L. Eötvös University, P.O. Box 32, H-1518 Budapest 112, Hungary.
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 May 15;879(17-18):1241-69. doi: 10.1016/j.jchromb.2011.01.027. Epub 2011 Feb 4.
An overview is presented on the advancement of the two most frequently used derivatization protocols applying the o-phthalaldehyde (OPA)-thiol and the fluorenylmethyloxycarbonyl (FMOC) chloride reagents, prior to the high performance liquid chromatographic analysis of amino acids. This review pays special attention (i) to the blank value, to the composition, to the stability and to the life time of the reagents, (ii) to the optimum pH conditions for the interactions and derivatives' elutions, (iii) to the characteristics and behavior of those amino acids which might provide more than one derivative correlating with the molar ratios of the reagent to the reactants, and (iv) on the practical applicability of the optimized protocols.
本文概述了在高效液相色谱分析氨基酸之前，应用邻苯二醛（OPA）-巯基和芴甲氧羰基（FMOC）氯两种最常用衍生化方法的进展。本综述特别关注（i）试剂的空白值、组成、稳定性和寿命，（ii）相互作用和衍生物洗脱的最佳 pH 条件，（iii）可能与试剂与反应物的摩尔比相关的生成一种以上衍生物的氨基酸的特性和行为，以及（iv）优化方案的实际适用性。
