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野生动物分子法庭科学:利用 STR 分析和贝叶斯分配检验鉴定撒丁岛野生绵羊。

Wildlife molecular forensics: identification of the Sardinian mouflon using STR profiling and the Bayesian assignment test.

机构信息

Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana, Via Tancia 21, 02100 Rieti, Italy.

出版信息

Forensic Sci Int Genet. 2011 Aug;5(4):345-9. doi: 10.1016/j.fsigen.2011.01.012. Epub 2011 Mar 2.

DOI:10.1016/j.fsigen.2011.01.012
PMID:21371958
Abstract

A forensic short tandem repeat (STR) typing test using a population database was developed to investigate an instance of poaching on the protected Sardinian mouflon. The case study involves a suspected poacher found in possession of a carcass, which he claimed was that of a sheep from his flock and had died accidentally. His claim was refuted by the molecular forensic analyses as DNA typing and the Bayesian assignment test revealed the carcass to be mouflon-derived; the genetic profile of the carcass matched also that of additional trace evidence collected by forestry officers at the scene of the kill. The matching evidence led to the poacher being charged with the illegal harvest of protected wildlife. Molecular techniques, in combination with a reference population database, and the appropriate statistical evaluation of genetic information, are fundamental to wildlife forensics. This approach allows DNA testing to be accepted in court as submissible evidence in the fight against poaching and other crimes involving wildlife.

摘要

采用群体数据库的法医短串联重复序列(STR)分型检测,被开发用于调查一起发生在受保护的撒丁岛野生绵羊禁猎区的偷猎案件。该案例研究涉及一名涉嫌偷猎者被发现持有一具尸体,他声称这是他羊群中的一只绵羊,意外死亡。但分子法医分析——DNA 分型和贝叶斯分配测试,推翻了他的说法,结果表明这具尸体来自野生绵羊;尸体的基因谱也与林业官员在杀戮现场收集的其他微量证据相匹配。匹配的证据导致该偷猎者被指控非法猎捕受保护的野生动物。分子技术,结合参考群体数据库,以及对遗传信息的适当统计评估,是野生动物法医学的基础。这种方法使 DNA 测试能够被法庭接受,作为打击偷猎和其他涉及野生动物犯罪的可接受证据。

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