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DNA定量系统、扩展STR分型多重试剂盒及基因分型数据库解决方案的发育验证

Developmental Validation of DNA Quantitation System, Extended STR Typing Multiplex, and Database Solutions for Genotyping.

作者信息

Vankova Lenka, Alaverdyan Johana, Vanek Daniel

机构信息

Institute for Environmental Sciences, Charles University Prague, Benatska 2, 12800 Prague, Czech Republic.

Forensic DNA Service, Budinova 2, 18081 Prague, Czech Republic.

出版信息

Life (Basel). 2025 Apr 17;15(4):664. doi: 10.3390/life15040664.

DOI:10.3390/life15040664
PMID:40283218
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12028859/
Abstract

This study describes the development of a species determination/DNA quantification system called Pleo Qplex and an individual identification STR multiplex called Pleo STRplex using samples. Pleo Qplex enables us to measure the quantity of extracted nuclear and mitochondrial DNA and detect the presence of co-purified inhibitors. Pleo STRplex, consisting of seven loci, enables the determination of the DNA profile from a sample of based on the analysis of short tandem repeats (STRs). The Pleo STRplex provides additional loci on top of previously published STR loci in Ptig STRplex and contains a specific STR marker that confirms . An allelic ladder of all STR markers was prepared to enable reliable allele calling. The STR loci can also be used to type the DNA of other members of the genus Panthera. The work on the resulting STR profiles is performed using GenoProof Suite, which offers databasing, matching, and relationship analysis.

摘要

本研究描述了一种名为Pleo Qplex的物种鉴定/DNA定量系统以及一种名为Pleo STRplex的个体识别STR复合扩增体系的开发，该体系使用了样本。Pleo Qplex使我们能够测量提取的核DNA和线粒体DNA的量，并检测共纯化抑制剂的存在。由七个位点组成的Pleo STRplex能够基于短串联重复序列（STR）分析从样本中确定DNA图谱。Pleo STRplex在先前发表的Ptig STRplex中的STR位点之上提供了额外的位点，并包含一个确认……的特定STR标记。制备了所有STR标记的等位基因阶梯以实现可靠的等位基因分型。这些STR位点也可用于对豹属其他成员的DNA进行分型。所得STR图谱的分析工作使用GenoProof Suite进行，该软件提供数据库建立、匹配和亲缘关系分析。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b25/12028859/d5110fe73009/life-15-00664-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b25/12028859/33c316dcec57/life-15-00664-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b25/12028859/b9459258d324/life-15-00664-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b25/12028859/0a1a876b292a/life-15-00664-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b25/12028859/6674b6614cc4/life-15-00664-g005.jpg
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