Leach Michelle K, Feng Zhang-Qi, Gertz Caitlyn C, Tuck Samuel J, Regan Tara M, Naim Youssef, Vincent Andrea M, Corey Joseph M
Department of Biomedical Engineering, University of Michigan, USA.
J Vis Exp. 2011 Feb 15(48):2389. doi: 10.3791/2389.
Electrospinning is a technique for producing micro- to nano-scale fibers. Fibers can be electrospun with varying degrees of alignment, from highly aligned to completely random. In addition, fibers can be spun from a variety of materials, including biodegradable polymers such as poly-L-lactic acid (PLLA). These characteristics make electrospun fibers suitable for a variety of scaffolding applications in tissue engineering. Our focus is on the use of aligned electrospun fibers for nerve regeneration. We have previously shown that aligned electrospun PLLA fibers direct the outgrowth of both primary sensory and motor neurons in vitro. We maintain that the use of a primary cell culture system is essential when evaluating biomaterials to model real neurons found in vivo as closely as possible. Here, we describe techniques used in our laboratory to electrospin fibrous scaffolds and culture dorsal root ganglia explants, as well as dissociated sensory and motor neurons, on electrospun scaffolds. However, the electrospinning and/or culture techniques presented here are easily adapted for use in other applications.
静电纺丝是一种生产微米至纳米级纤维的技术。纤维可以通过静电纺丝制成不同程度排列的，从高度排列到完全随机排列。此外，纤维可以由多种材料纺制而成，包括可生物降解的聚合物，如聚-L-乳酸（PLLA）。这些特性使静电纺丝纤维适用于组织工程中的各种支架应用。我们的重点是使用排列的静电纺丝纤维促进神经再生。我们之前已经表明，排列的静电纺丝PLLA纤维在体外引导初级感觉神经元和运动神经元的生长。我们认为，在评估生物材料时，使用原代细胞培养系统对于尽可能紧密地模拟体内真实神经元至关重要。在这里，我们描述了我们实验室用于静电纺丝纤维支架以及在静电纺丝支架上培养背根神经节外植体以及解离的感觉神经元和运动神经元的技术。然而，这里介绍的静电纺丝和/或培养技术很容易适用于其他应用。
