Maples Thomas, Rothenfluh Adrian
Department of Psychiatry, University of Texas Southwestern Medical Center, USA.
J Vis Exp. 2011 Feb 19(48):2541. doi: 10.3791/2541.
Low doses of ethanol cause flies to become hyperactive, while high doses are sedating. The sensitivity to ethanol-induced sedation of a given fly strain is correlated with that strain s ethanol preference, and therefore sedation is a highly relevant measure to study the genetics of alcohol responses and drinking. We demonstrate a simple way to expose flies to ethanol and measure its intoxicating effects. The assay we describe can determine acute sensitivity, as well as ethanol tolerance induced by repeat exposure. It does not require a technically involved setup, and can therefore be applied in any laboratory with basic fly culture tools.
低剂量乙醇会使果蝇变得多动，而高剂量则会起到镇静作用。给定果蝇品系对乙醇诱导的镇静作用的敏感性与该品系对乙醇的偏好相关，因此镇静是研究酒精反应和饮酒遗传学的高度相关指标。我们展示了一种让果蝇接触乙醇并测量其醉态影响的简单方法。我们所描述的测定方法可以确定急性敏感性以及重复接触所诱导的乙醇耐受性。它不需要复杂的技术设置，因此可以在任何拥有基本果蝇培养工具的实验室中应用。
