Zer Shir, Ryvkin Julia, Wilner Harel J, Zak Hila, Shmueli Anat, Shohat-Ophir Galit
The Mina & Everard Goodman Faculty of Life Sciences, The Leslie and Susan Gonda Multidisciplinary Brain Research Center, Bar-Ilan University.
The Mina & Everard Goodman Faculty of Life Sciences, The Leslie and Susan Gonda Multidisciplinary Brain Research Center, Bar-Ilan University;
J Vis Exp. 2016 Dec 15(118):54910. doi: 10.3791/54910.
We describe a protocol for measuring ethanol self-administration in fruit flies (Drosophila melanogaster) as a proxy for changes in reward states. We demonstrate a simple way to tap into the fly reward system, modify experiences related to natural reward, and use voluntary ethanol consumption as a measure for changes in reward states. The approach serves as a relevant tool to study the neurons and genes that play a role in experience-mediated changes of internal state. The protocol is composed of two discrete parts: exposing the flies to rewarding and nonrewarding experiences, and assaying voluntary ethanol consumption as a measure of the motivation to obtain a drug reward. The two parts can be used independently to induce the modulation of experience as an initial step for further downstream assays or as an independent two-choice feeding assay, respectively. The protocol does not require a complicated setup and can therefore be applied in any laboratory with basic fly culture tools.
我们描述了一种用于测量果蝇（黑腹果蝇）乙醇自我给药的方案，以此作为奖励状态变化的替代指标。我们展示了一种简单的方法来接入果蝇奖励系统，改变与自然奖励相关的体验，并将自愿乙醇消耗用作奖励状态变化的衡量指标。该方法是研究在内部状态的经验介导变化中起作用的神经元和基因的相关工具。该方案由两个独立部分组成：让果蝇经历奖励和无奖励体验，以及测定自愿乙醇消耗作为获得药物奖励动机的指标。这两个部分可分别独立用于诱导体验调节，作为进一步下游分析的初始步骤，或作为独立的二选一进食分析。该方案不需要复杂的设置，因此可以在任何配备基本果蝇培养工具的实验室中应用。
