Nakajima H, Yamasaki T, Noguchi T, Tanaka T, Kono N, Tarui S
Second Department of Internal Medicine, Osaka University Medical School, Japan.
Biochem Biophys Res Commun. 1990 Jan 30;166(2):637-41. doi: 10.1016/0006-291x(90)90856-i.
Three mRNA species for human muscle phophofructokinase containing heterogeneous 5' untranslated sequences were identified through cDNA cloning. Type A mRNA was essentially the same as that reported previously (Nakajima, H., et al. (1987) FEBS Lett. 223, 113). Type B mRNA was considered to be the major gene product, which contained an extra non-coding sequence within the 5' untranslated region of type A mRNA. Amplification of mRNA by polymerase chain reaction revealed that types A and B mRNAs shared a common precursor RNA, and were alternatively spliced. Type C mRNA, homologous to the cDNA sequence from a placenta library (Sharma, P. M. et al., (1989) Gene 77, 177), was considered to be under the control of an alternative promotor.
通过cDNA克隆鉴定出了三种含有异质5'非翻译序列的人肌肉磷酸果糖激酶的mRNA种类。A型mRNA与先前报道的基本相同（中岛，H.等人（1987年），《欧洲生物化学学会联合会快报》223，113）。B型mRNA被认为是主要的基因产物，它在A型mRNA的5'非翻译区内包含一个额外的非编码序列。通过聚合酶链反应对mRNA进行扩增表明，A型和B型mRNA共享一个共同的前体RNA，并且是可变剪接的。C型mRNA与来自胎盘文库的cDNA序列同源（夏尔马，P.M.等人，（1989年），《基因》77，177），被认为受另一个启动子的控制。
