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人类磷酸果糖激酶-M和-L基因在表达及RNA可变剪接方面的组织特异性

Tissue specificity in expression and alternative RNA splicing of human phosphofructokinase-M and -L genes.

作者信息

Nakajima H, Kono N, Yamasaki T, Hamaguchi T, Hotta K, Kuwajima M, Noguchi T, Tanaka T, Tarui S

机构信息

Second Department of Internal Medicine, Osaka University Medical School, Japan.

出版信息

Biochem Biophys Res Commun. 1990 Dec 31;173(3):1317-21. doi: 10.1016/s0006-291x(05)80931-3.

DOI:10.1016/s0006-291x(05)80931-3
PMID:2148476
Abstract

Mode of the expression of phosphofructokinase (PFK) -M and -L genes was examined in various human tissues including muscle, placenta, liver, kidney, pancreas, stomach and reticulocytes. The gross level of mRNA expression of PFK-M and -L genes was estimated by Northern analysis. Polymerase chain reaction was used to detect mRNA expressed at low levels in these tissues. Tissue-specific expression of alternatively spliced PFK-M gene transcripts was also determined by polymerase chain reaction. The results indicated that alternative splicing of PFK-M gene transcripts was controlled in a tissue-specific manner.

摘要

在包括肌肉、胎盘、肝脏、肾脏、胰腺、胃和网织红细胞在内的各种人体组织中，研究了磷酸果糖激酶（PFK）-M和-L基因的表达模式。通过Northern分析估计PFK-M和-L基因的mRNA表达总体水平。采用聚合酶链反应检测这些组织中低水平表达的mRNA。还通过聚合酶链反应确定了选择性剪接的PFK-M基因转录本的组织特异性表达。结果表明，PFK-M基因转录本的选择性剪接是以组织特异性方式控制的。

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