Valdez B C, Chen Z, Sosa M G, Younathan E S, Chang S H
Department of Biochemistry, Louisiana State University, Baton Rouge 70803.
Gene. 1989 Mar 15;76(1):167-9. doi: 10.1016/0378-1119(89)90019-x.
A 6-phosphofructo-1-kinase-coding gene (pfk) has been purified from a human genomic library cloned in the lambda EMBL4 phage vector. This clone contains the nontranslated 5' flanking region of the human muscle pfk gene. Comparison of the nucleotide sequence determined by us with that of the human muscle pfk cDNA [Nakajima et al., FEBS Lett. 223 (1987) 113-116] indicates the presence of an additional intron extending from nucleotide (nt) -97 to -9 upstream of the ATG start codon. Furthermore, the human muscle pfk gene is more AT-rich than the rabbit gene. The available sequence of the two cDNAs shows 256 nt differences. Surprisingly, 71% of these sites are A's and T's in the human cDNA and C's and G's in the rabbit gene.
已从克隆于λEMBL4噬菌体载体的人类基因组文库中纯化出一个6-磷酸果糖-1-激酶编码基因（pfk）。该克隆包含人类肌肉pfk基因的非翻译5'侧翼区。我们测定的核苷酸序列与人类肌肉pfk cDNA的核苷酸序列[中岛等人，《欧洲生物化学学会联合会快报》223（1987）113 - 116]比较表明，在ATG起始密码子上游从核苷酸（nt）-97至-9处存在一个额外的内含子。此外，人类肌肉pfk基因比兔基因富含更多的AT。两个cDNA的可用序列显示有256个核苷酸差异。令人惊讶的是，这些位点中有71%在人类cDNA中是A和T，而在兔基因中是C和G。
