Department of Chemistry & Maurice Morton Institute of Polymer Science, The University of Akron, Akron, OH 44325, USA.
Org Biomol Chem. 2011 Apr 21;9(8):2878-84. doi: 10.1039/c0ob01061h. Epub 2011 Mar 3.
A novel fluorescent system has been assembled by using ATP, surfactant, and a squaraine dye in an aqueous buffer solution. In the system, a cationic surfactant such as cetyl trimethyl ammonium bromide (CTAB) forms a sphere-like micelle, whose positive charge at the surface of the micelle attracts the negatively charged ATP to form a unique organized nanostructure. Such an organized system is shown to interact with the squaraine dye (SQ) to perturb its aggregate structure, thereby generating the optical response. The nanostructure of the assembly has been characterized by dynamic light-scattering (DLS) and atomic force microscopy (AFM). The unique feature of the developed sensing system is that the analytes ATP form part of the assembly structure. The system utilizes forces such as electrostatic interaction and π-π stacking of the aromatic segment of ATP and SQ to achieve the selective detection of ATP.
一个新型的荧光体系由三磷酸腺苷（ATP）、表面活性剂和方酸染料在水性缓冲溶液中组装而成。在该体系中，十六烷基三甲基溴化铵（CTAB）等阳离子表面活性剂形成类球形胶束，胶束表面的正电荷吸引带负电荷的 ATP 形成独特的有序纳米结构。这种有序体系与方酸染料（SQ）相互作用，从而扰乱其聚集结构，产生光学响应。该组装体的纳米结构通过动态光散射（DLS）和原子力显微镜（AFM）进行了表征。所开发的传感体系的独特之处在于，分析物 ATP 构成了组装结构的一部分。该体系利用静电相互作用和 ATP 及 SQ 的芳香片段的 π-π 堆积等力来实现对 ATP 的选择性检测。
