Cuomo Francesca, Lopez Francesco, Angelico Ruggero, Colafemmina Giuseppe, Ceglie Andrea
Consorzio Interuniversitario per lo sviluppo dei Sistemi a Grande Interfase (CSGI), c/o Department of Food Technology (DISTAAM), Università del Molise, I-86100 Campobasso, Italy.
Colloids Surf B Biointerfaces. 2008 Jul 15;64(2):184-93. doi: 10.1016/j.colsurfb.2008.01.020. Epub 2008 Feb 3.
In this paper a micellar interface, constituted by the cationic surfactant CTAB, in presence of 1,2-epoxydodecane and nucleotides was used for catanionic multi-lamellar vesicles (MLVs) formation. The micellar solution of CTAB is able to disperse the 1,2 epoxydodecane in the micellar core promoting the reaction of this reagent with the nucleotide attracted by the positive surface charge of the micellar aggregates. The alkylation of AMP and UMP nucleotides leads to the synthesis of nucleolipids. The behaviour of the supramolecular structures formed depends on the starting reagents (AMP, UMP and AMP+UMP) and on the assembly capabilities of the products. In particular nucleotides and nucleotides derivatives interaction effects are evaluated during the multi-lamellar vesicles formation. NMR spectroscopy and UV-vis measurements performed on MLVs showed strong aryl interactions. Interestingly, NMR spectra revealed prevailing stacking interactions between complementary nucleolipids. The assembly of complementary nucleotides affects the course of the reaction during the MLVs formation. Moreover the MLVs supramolecular stability has been tested by means of turbidity and UV-vis measurements. In particular, an enhanced stability has been found in systems prepared with complementary nucleotides confirming that in these systems the self-assembly process is influenced by nucleolipids interactions. Furthermore by following the hypocromic effect during the micellar catalysis, we showed that even in the earlier stages of the reaction significant differences are detectable.
在本文中，由阳离子表面活性剂十六烷基三甲基溴化铵（CTAB）构成的胶束界面，在1,2 - 环氧十二烷和核苷酸存在的情况下，被用于形成阴阳离子多层囊泡（MLV）。CTAB的胶束溶液能够将1,2 - 环氧十二烷分散在胶束核心中，促进该试剂与被胶束聚集体正表面电荷吸引的核苷酸发生反应。AMP和UMP核苷酸的烷基化导致核脂质的合成。所形成的超分子结构的行为取决于起始试剂（AMP、UMP和AMP + UMP）以及产物的组装能力。特别是在多层囊泡形成过程中评估了核苷酸和核苷酸衍生物的相互作用效果。对MLV进行的核磁共振光谱和紫外 - 可见光谱测量显示出强烈的芳基相互作用。有趣的是，核磁共振光谱揭示了互补核脂质之间主要的堆积相互作用。互补核苷酸的组装会影响MLV形成过程中的反应进程。此外，通过浊度和紫外 - 可见光谱测量测试了MLV的超分子稳定性。特别是，在用互补核苷酸制备的系统中发现了增强的稳定性，这证实了在这些系统中自组装过程受核脂质相互作用的影响。此外，通过追踪胶束催化过程中的减色效应，我们表明即使在反应的早期阶段也能检测到显著差异。
