Department of Internal Medicine, National Cheng Kung University Medical Center, Tainan, Taiwan.
Mol Carcinog. 2011 Oct;50(10):751-9. doi: 10.1002/mc.20753. Epub 2011 Mar 3.
We tested whether cagL amino acid sequence polymorphisms of Helicobacter pylori correlated to clinico-histological outcomes and gastric α5β1 integrin expressions. One hundred forty five patients with H. pylori infection and 47 noninfected controls were enrolled to check gastric integrin α5β1 intensities topographically. The collected isolates were screened for cagL-genotype by polymerase chain reaction (PCR), and assessed for amino acid sequence polymorphisms using sequence translation. Our H. pylori isolates were predominantly (98.6%) cagL-genopositive, 95.8% of which had the RGD motif in their amino acid sequences. The isolates from the gastric cancer (GCA) patients indicated a higher rate of amino acid sequence polymorphisms-Y58 and E59-than those of the non-GCA patients (P < 0.05). The polymorphisms as Y58E59 noted with increased risk of GCA up to 4.6-fold (95%CI: 1.8-11.9). H. pylori-infected patients had higher integrin α5β1 than noninfected patients (P < 0.05). Furthermore, cagL-Y58E59 H. pylori infection predisposed an upward shift in integrin α5β1 (P = 0.007) in the corpus, leading to more severe corpus chronic inflammation (P < 0.05). H. pylori CagL amino acid polymorphisms like Y58E59 correlate with a higher risk of GCA, and may regulate a corpus shift of gastric integrin α5β1 to lead to severe corpus gastritis during gastric carcinogenesis.
我们检测了幽门螺杆菌 cagL 氨基酸序列多态性是否与临床病理结果和胃α5β1 整合素表达相关。招募了 145 例幽门螺杆菌感染患者和 47 例非感染对照者，以检查胃整合素α5β1 的拓扑强度。通过聚合酶链反应（PCR）筛选收集的分离株的 cagL 基因型，并通过序列翻译评估氨基酸序列多态性。我们的幽门螺杆菌分离株主要为 cagL-阳性（98.6%），其中 95.8%的分离株在其氨基酸序列中具有 RGD 基序。胃癌（GCA）患者的分离株的氨基酸序列多态性-Y58 和 E59 发生率高于非 GCA 患者（P<0.05）。多态性如 Y58E59 与 GCA 的风险增加高达 4.6 倍（95%CI：1.8-11.9）。感染幽门螺杆菌的患者的整合素α5β1 高于未感染的患者（P<0.05）。此外，cagL-Y58E59 幽门螺杆菌感染使整合素α5β1 在胃体向上移位（P=0.007），导致更严重的胃体慢性炎症（P<0.05）。幽门螺杆菌 CagL 氨基酸多态性如 Y58E59 与 GCA 的风险增加相关，并且可能调节胃整合素α5β1 的胃体移位，导致胃癌发生过程中的严重胃体胃炎。
