Wang Yan-chun, Cheng Yu-he, Ma Jun, Gan Shui-yong, Wang Shu-ju, Zhou Hua, Du Yan-jun, Yang Min, Shen Feng
Hubei University of Chinese Medicine, Wuhan 430061, China.
Zhen Ci Yan Jiu. 2010 Dec;35(6):415-21.
To observe the influence of electroacupuncture (EA) of "Fengfu" (GV16) and "Taichong" (LR3) on morphological changes and apoptotic percentage of substantia nigra cells so as to explore its underlying mechanism in relieving Parkinson's disease.
Forty Wistar rats were randomly divided into normal control, sham-operation (sham), model and EA groups, with 10 rats in each. The Parkinson's disease model was established by micro-injection of 6-hydroxyl-dopamine (6-OHDA) into the right striatum. EA (2 Hz, 1 mA) was applied to "Fengfu"(GV16) and "Taichong" (LR3) for 30 min, once daily for 2 weeks. HE staining, Nissl staining and immunohistochemical staining were conducted for observing the morphological changes of substantia nigra pars compacta (SNc) and striatum, and the tyrosine hydroxylase(TH) immuno-reaction (IR) positive neurons and nerve fibers. The apoptosis was detected by flow cytometry.
Compared to the normal control and sham groups, the total number of TH-IR positive neurons and Nissl-stained cells, and OD value of TH-IR positive nerve fibers in the SNc on the 6-OHDA-lesioned side were significantly lower in the model group (P < 0.01). Compared with the model group, the aforementioned 3 indexes of the EA group were increased obviously (P < 0.01). The percentage of apoptosis of SNc on the 6-OHDA-lesioned side was obviously lower in the EA group than in the model group (P < 0.01), but still obviously higher than in the normal group and sham group (P < 0.01).
Electroacupuncture therapy can significantly increase the number of neurons of substantia nigra and the density of striatum nerve fibers,and reduce the apoptotic percentage of substantia nigra cells in the Parkinsonian rats, which may contribute to its effect in relieving Parkinson's disease.
观察电针“风府”（GV16）、“太冲”（LR3）对帕金森病大鼠黑质细胞形态学变化及凋亡率的影响，以探讨其治疗帕金森病的潜在机制。
将40只Wistar大鼠随机分为正常对照组、假手术组、模型组和电针组，每组10只。采用微量注射6-羟基多巴胺（6-OHDA）至右侧纹状体的方法制备帕金森病模型。电针“风府”（GV16）、“太冲”（LR3），频率2Hz，强度1mA，每次30分钟，每日1次，连续2周。采用苏木精-伊红（HE）染色、尼氏染色及免疫组织化学染色观察黑质致密部（SNc）和纹状体的形态学变化，以及酪氨酸羟化酶（TH）免疫反应（IR）阳性神经元和神经纤维。采用流式细胞术检测细胞凋亡情况。
与正常对照组和假手术组比较，模型组6-OHDA损伤侧SNc区TH-IR阳性神经元总数、尼氏染色细胞数及TH-IR阳性神经纤维光密度值均明显降低（P<0.01）。与模型组比较，电针组上述3项指标均明显升高（P<0.01）。电针组6-OHDA损伤侧SNc区细胞凋亡率明显低于模型组（P<0.01），但仍明显高于正常组和假手术组（P<0.01）。
电针治疗可明显增加帕金森病大鼠黑质神经元数量和纹状体神经纤维密度，降低黑质细胞凋亡率，这可能是其治疗帕金森病的作用机制之一。
