Department of Chemistry, King Fahd University of Petroleum and Minerals, KFUPM Box 1059, Dhahran 31261, Saudi Arabia.
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 May 15;879(17-18):1180-8. doi: 10.1016/j.jchromb.2011.02.009. Epub 2011 Feb 13.
Liquid phase microextraction (LPME), especially hollow fiber liquid-phase microextraction (HF-LPME), and dispersive liquid-liquid microextraction (DLLME) offer high enrichments of target analytes in a single step. The analytical usefulness of these techniques is significantly enhanced by coupling them with suitable derivatization methods. Due to their simplicity, diverse bioanalytical applications have recently been reported. This review focuses on the recent developments of the combined LPME (mainly HF-LPME and single drop microextraction (SDME)) and DLLME techniques with derivatization for the analysis of biological samples. A broad range of sample matrices such as urine, blood, plasma and human hair samples with various derivatization methods for polar or ionizable organic compounds will be considered. These techniques can also be extended to the determination of trace metal ions, such as the heavy metal ions (Hg, Pb, and Co) and Se. Future trends of the techniques will also be discussed.
液相微萃取（LPME），尤其是中空纤维液相微萃取（HF-LPME）和分散液相微萃取（DLLME），可在单一步骤中对目标分析物进行高浓度富集。通过将这些技术与合适的衍生化方法结合使用，可以显著提高它们的分析有用性。由于其简单性，最近已经报道了多种生物分析应用。本文综述了近年来将 LPME（主要是 HF-LPME 和单滴微萃取（SDME））与衍生化技术相结合，用于分析生物样品的最新进展。将考虑各种衍生化方法对尿液、血液、血浆和人发等多种样品基质中极性或可离子化有机化合物的衍生化。这些技术还可以扩展到痕量金属离子（如重金属离子（Hg、Pb 和 Co）和 Se）的测定。还将讨论这些技术的未来发展趋势。
