Department of Radiotherapy and Radiation Oncology, University of Leipzig, Stephanstrasse 21, 04103 Leipzig, Germany.
Mutat Res. 2011 May 10;709-710:32-9. doi: 10.1016/j.mrfmmm.2011.02.007. Epub 2011 Mar 3.
Ionizing irradiation could act directly on immune cells and may induce bystander effects mediated by soluble factors that are released by the irradiated cells. This is the first study analyzing both the direct effect of low dose ionizing radiation (LDIR) on the maturation and cytokine release of human dendritic cells (DCs) and the functional consequences for co-cultured T-cells. We showed that irradiation of DC-precursors in vitro does not influence surface marker expression or cytokine profile of immature DCs nor of mature DCs after LPS treatment. There was no difference of single dose irradiation versus fractionated irradiation protocols on the behavior of the mature DCs. Further, the low dose irradiation did not change the capacity of the DCs to stimulate T-cell proliferation. But the irradiation of the co-culture of DCs and T-cells revealed significantly lower proliferation of T-cells with higher doses. Summarizing the data from approx. 50 DC preparations there is no significant effect of low dose ionizing irradiation on the cytokine profile, surface marker expression and maturation of DCs in vitro although functional consequences cannot be excluded.
电离辐射可以直接作用于免疫细胞，并可能通过被照射细胞释放的可溶性因子诱导旁观者效应。这是第一项分析低剂量电离辐射（LDIR）对人树突状细胞（DC）成熟和细胞因子释放的直接影响以及对共培养 T 细胞的功能后果的研究。我们表明，体外照射 DC 前体不会影响未成熟 DC 的表面标志物表达或细胞因子谱，也不会影响 LPS 处理后的成熟 DC。在成熟 DC 的行为上，单次剂量照射与分次照射方案没有区别。此外，低剂量照射不会改变 DC 刺激 T 细胞增殖的能力。但是，当共培养 DC 和 T 细胞时，随着照射剂量的增加，T 细胞的增殖明显减少。总结约 50 个 DC 制剂的数据，尽管不能排除功能后果，但低剂量电离照射对体外 DC 的细胞因子谱、表面标志物表达和成熟没有显著影响。
