Buschard K, Damsbo P, Röpke C
Bartholin Institute, Kommunehospitalet, Copenhagen, Denmark.
Diabet Med. 1990 Feb;7(2):132-6. doi: 10.1111/j.1464-5491.1990.tb01347.x.
Peripheral lymphocytes from 13 Type 1 diabetic patients (age 28 +/- 11 (+/- SD) years) were studied at diagnosis, and 1 month and 7 months later. The lymphocytes were labelled with phycoerythrin-conjugated anti-HLA-DR antibody and/or fluorescein-conjugated OKT4 or OKT8 antibody, and then studied using a double channel flow-cytometer. The overall percentage of activated T-lymphocytes (CD4+ + CD8+ cells) was (mean +/- SE) 3.1 +/- 0.5% at diagnosis, 2.1 +/- 0.3% at 1 month and 2.2 +/- 0.5% at 7 months test. The percentages at diagnosis and 1 month were higher than in 12 healthy control subjects (1.0 +/- 0.2%, p less than 0.001 and p less than 0.01, respectively). Among the CD4+ cells the percentage of activated cells fell from diagnosis (2.8 +/- 0.7%), to 1 month (1.5 +/- 0.3%, p less than 0.05), and 7 months (1.5 +/- 0.4%, p less than 0.05), whereas among the CD8+ cells they remained unchanged (3.3 +/- 0.6, 2.5 +/- 0.5 and 3.1 +/- 0.6%). The percentage of activated CD4+ cells at diagnosis, and of CD8+ cells at all times, were higher (p less than 0.02) than in control subjects (CD4+, 0.8 +/- 0.2%; CD8+, 1.2 +/- 0.2%). In conclusion, the activated T-lymphocytes present in Type 1 diabetic patients at diagnosis are equally increased among CD4+ and CD8+ cells, but after 7 months the increase is predominantly in CD8+ cells.
对13名1型糖尿病患者（年龄28±11（±标准差）岁）诊断时、1个月后和7个月后的外周淋巴细胞进行了研究。淋巴细胞用藻红蛋白偶联抗HLA - DR抗体和/或异硫氰酸荧光素偶联OKT4或OKT8抗体进行标记，然后使用双通道流式细胞仪进行研究。诊断时活化T淋巴细胞（CD4 + + CD8 +细胞）的总体百分比为（平均值±标准误）3.1±0.5%，1个月时为2.1±0.3%，7个月检测时为2.2±0.5%。诊断时和1个月时的百分比高于12名健康对照者（分别为1.0±0.2%，p＜0.001和p＜0.01）。在CD4 +细胞中，活化细胞的百分比从诊断时的（2.8±0.7%）降至1个月时的（1.5±0.3%，p＜0.05）和7个月时的（1.5±0.4%，p＜0.05），而在CD8 +细胞中则保持不变（3.3±0.6、2.5±0.5和3.1±0.6%）。诊断时活化CD4 +细胞的百分比以及所有时间点CD8 +细胞的百分比均高于对照者（p＜0.02）（CD4 +，0.8±0.2%；CD8 +，1.2±0.2%）。总之，1型糖尿病患者诊断时存在的活化T淋巴细胞在CD4 +和CD8 +细胞中均有同等程度增加，但7个月后主要在CD8 +细胞中增加。
