Sharma S K, Banyal H S
Department of Biosciences, Himachal Pradesh University, Shimla, India.
Parasitol Int. 2011 Sep;60(3):321-3. doi: 10.1016/j.parint.2011.02.006. Epub 2011 Mar 4.
Plasmodium berghei contained 0.454±0.031 U/mg of glutathione synthetyase (GS). GS was purified using solid ammonium sulfate and Sephadex G-200 from P. berghei infected mouse erythrocytes. SDS-PAGE showed purified GS as a single band protein of 70 kDa and its Km for γ-glutamylcysteine, glycine and ATP being 0.33 mM, 8.3 mM and 0.43 mM respectively with noncompetitive inhibition by GSH. The malaria parasite enzyme was optimally active at 37°C and pH 8.0-8.5. Heavy metals significantly inhibited parasite GS activity.
伯氏疟原虫含有0.454±0.031 U/mg的谷胱甘肽合成酶（GS）。使用固体硫酸铵和葡聚糖凝胶G-200从感染伯氏疟原虫的小鼠红细胞中纯化GS。SDS-PAGE显示纯化的GS为一条70 kDa的单带蛋白，其对γ-谷氨酰半胱氨酸、甘氨酸和ATP的Km分别为0.33 mM、8.3 mM和0.43 mM，受谷胱甘肽非竞争性抑制。疟原虫酶在37°C和pH 8.0 - 8.5时活性最佳。重金属显著抑制疟原虫GS活性。
