利用微刻蚀技术生产和筛选具有增强蛋白表达的毕赤酵母菌株。
Generation and screening of Pichia pastoris strains with enhanced protein production by use of microengraving.
机构信息
Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave., Building 76-253, Cambridge, MA 02139, USA.
出版信息
Appl Environ Microbiol. 2011 May;77(9):3154-6. doi: 10.1128/AEM.00104-11. Epub 2011 Mar 4.
Abstract
The selection of highly productive cell lines remains a key step for manufacturing therapeutic proteins. Microengraving was used to screen chemically mutagenized populations of Pichia pastoris for increased production of an Fc fragment. Clones retrieved following three rounds of mutagenesis yielded titers 2.65-fold greater than those of the parental strain.
摘要
筛选高产细胞系仍然是生产治疗性蛋白的关键步骤。微刻蚀技术被用于筛选经过化学诱变的巴斯德毕赤酵母（Pichia pastoris）种群，以提高 Fc 片段的产量。经过三轮诱变获得的克隆的产量比亲本菌株高 2.65 倍。
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