实现高通量单细胞蛋白质表征的微流控系统的开发。
Development of Microfluidic Systems Enabling High-Throughput Single-Cell Protein Characterization.
作者信息
Fan Beiyuan, Li Xiufeng, Chen Deyong, Peng Hongshang, Wang Junbo, Chen Jian
机构信息
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China.
School of Science, Minzu University of China, Beijing 100081, China.
出版信息
Sensors (Basel). 2016 Feb 16;16(2):232. doi: 10.3390/s16020232.
This article reviews recent developments in microfluidic systems enabling high-throughput characterization of single-cell proteins. Four key perspectives of microfluidic platforms are included in this review: (1) microfluidic fluorescent flow cytometry; (2) droplet based microfluidic flow cytometry; (3) large-array micro wells (microengraving); and (4) large-array micro chambers (barcode microchips). We examine the advantages and limitations of each technique and discuss future research opportunities by focusing on three key performance parameters (absolute quantification, sensitivity, and throughput).
本文综述了微流控系统的最新进展，这些进展使得单细胞蛋白质的高通量表征成为可能。本综述涵盖了微流控平台的四个关键方面：（1）微流控荧光流式细胞术；（2）基于液滴的微流控流式细胞术；（3）大阵列微孔（微刻蚀）；（4）大阵列微腔（条形码微芯片）。我们研究了每种技术的优缺点，并通过关注三个关键性能参数（绝对定量、灵敏度和通量）来讨论未来的研究机会。
Zotero 插件