构建和应用米曲霉细胞色素 P450 单加氧酶功能文库。
Construction and application of a functional library of cytochrome P450 monooxygenases from the filamentous fungus Aspergillus oryzae.
机构信息
Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
出版信息
Appl Environ Microbiol. 2011 May;77(9):3147-50. doi: 10.1128/AEM.02491-10. Epub 2011 Mar 4.
Abstract
A functional library of cytochrome P450 monooxygenases from Aspergillus oryzae (AoCYPs) was constructed in which 121 isoforms were coexpressed with yeast NADPH-cytochrome P450 oxidoreductase in Saccharomyces cerevisiae. Using this functional library, novel catalytic functions of AoCYPs, such as catalytic potentials of CYP57B3 against genistein, were elucidated for the first time. Comprehensive functional screening promises rapid characterization of catalytic potentials and utility of AoCYPs.
摘要
构建了来自米曲霉（Aspergillus oryzae）的细胞色素 P450 单加氧酶的功能性文库，其中 121 种同工型与酵母 NADPH-细胞色素 P450 氧化还原酶在酿酒酵母中共同表达。利用这个功能性文库，首次阐明了 AoCYPs 的新催化功能，如 CYP57B3 对染料木黄酮的催化潜力。全面的功能筛选有望快速表征 AoCYPs 的催化潜力和用途。
相似文献
1
Construction and application of a functional library of cytochrome P450 monooxygenases from the filamentous fungus Aspergillus oryzae.构建和应用米曲霉细胞色素 P450 单加氧酶功能文库。
Appl Environ Microbiol. 2011 May;77(9):3147-50. doi: 10.1128/AEM.02491-10. Epub 2011 Mar 4.
2
Impacts of amino acid substitutions in fungal cytochrome P450 monooxygenase (CYP57B3) on the effective production of 3΄-hydroxygenistein.真菌细胞色素P450单加氧酶（CYP57B3）中氨基酸取代对3΄-羟基大豆黄素有效生产的影响。
FEMS Microbiol Lett. 2017 Jun 15;364(11). doi: 10.1093/femsle/fnx107.
3
Molecular characterization and isolation of cytochrome P450 genes from the filamentous fungus Aspergillus oryzae.从丝状真菌米曲霉中细胞色素 P450 基因的分子特征描述和分离。
Arch Microbiol. 2010 May;192(5):395-408. doi: 10.1007/s00203-010-0562-z. Epub 2010 Apr 1.
4
Novel 4-methyl-2-oxopentanoate reductase involved in synthesis of the Japanese sake flavor, ethyl leucate.参与日本清酒风味物质己酸乙酯合成的新型4-甲基-2-氧代戊酸还原酶。
Appl Microbiol Biotechnol. 2016 Apr;100(7):3137-45. doi: 10.1007/s00253-015-7182-0. Epub 2015 Nov 28.
5
Molecular evolution and characterization of fungal indoleamine 2,3-dioxygenases.真菌吲哚胺 2,3-双加氧酶的分子进化与特征分析。
J Mol Evol. 2011 Feb;72(2):160-8. doi: 10.1007/s00239-010-9412-5. Epub 2010 Dec 18.
6
Expression of Aspergillus oryzae alpha-amylase gene in Saccharomyces cerevisiae.米曲霉α-淀粉酶基因在酿酒酵母中的表达。
FEMS Microbiol Lett. 1993 Aug 15;112(1):119-23. doi: 10.1111/j.1574-6968.1993.tb06433.x.
7
Comparative functional characterization of a novel benzoate hydroxylase cytochrome P450 of Fusarium oxysporum.尖孢镰刀菌一种新型苯甲酸羟化酶细胞色素P450的比较功能表征
Enzyme Microb Technol. 2015 Mar;70:58-65. doi: 10.1016/j.enzmictec.2014.12.013. Epub 2014 Dec 31.
8
Cellular responses to the expression of unstable secretory proteins in the filamentous fungus Aspergillus oryzae.丝状真菌米曲霉中细胞对不稳定分泌蛋白表达的反应。
Appl Microbiol Biotechnol. 2017 Mar;101(6):2437-2446. doi: 10.1007/s00253-016-8086-3. Epub 2017 Jan 7.
9
P450 redox enzymes in the white rot fungus Phanerochaete chrysosporium: gene transcription, heterologous expression, and activity analysis on the purified proteins.白腐真菌糙皮侧耳中的 P450 氧化还原酶：基因转录、异源表达及纯蛋白活性分析。
Curr Microbiol. 2010 Oct;61(4):306-14. doi: 10.1007/s00284-010-9612-7. Epub 2010 Mar 11.
10
Optimization of yeast-expressed human liver cytochrome P450 3A4 catalytic activities by coexpressing NADPH-cytochrome P450 reductase and cytochrome b5.通过共表达NADPH-细胞色素P450还原酶和细胞色素b5优化酵母表达的人肝细胞色素P450 3A4的催化活性
Eur J Biochem. 1992 Jul 1;207(1):109-16. doi: 10.1111/j.1432-1033.1992.tb17027.x.
引用本文的文献
1
Functional expression and regulation of eukaryotic cytochrome P450 enzymes in surrogate microbial cell factories.真核细胞色素P450酶在替代微生物细胞工厂中的功能表达与调控
Eng Microbiol. 2022 Jan 19;2(1):100011. doi: 10.1016/j.engmic.2022.100011. eCollection 2022 Mar.
2
Pharmaceutical removal from wastewater by introducing cytochrome P450s into microalgae.通过向微藻中引入细胞色素 P450 来去除废水中的药物。
Microb Biotechnol. 2024 Jun;17(6):e14515. doi: 10.1111/1751-7915.14515.
3
Transposable elements impact the population divergence of rice blast fungus .转座元件影响稻瘟病菌的种群分歧。
mBio. 2024 May 8;15(5):e0008624. doi: 10.1128/mbio.00086-24. Epub 2024 Mar 27.
4
Biocatalyst collection and heterologous expression of sesquiterpene synthases from basidiomycetous fungi: Discovery of a novel sesquiterpene hydrocarbon.担子菌来源的倍半萜合酶的生物催化剂的收集和异源表达：新型倍半萜烃的发现。
Microb Biotechnol. 2023 Mar;16(3):632-644. doi: 10.1111/1751-7915.14204. Epub 2022 Dec 28.
5
Evolution and enrichment of CYP5035 in Polyporales: functionality of an understudied P450 family.多孔菌目 CYP5035 的进化和富集：一个研究较少的 P450 家族的功能。
Appl Microbiol Biotechnol. 2021 Sep;105(18):6779-6792. doi: 10.1007/s00253-021-11444-2. Epub 2021 Aug 30.
6
Altering the Regioselectivity of Cytochrome P450 BM3 Variant M13 toward Genistein through Protein Engineering and Variation of Reaction Conditions.通过蛋白质工程和反应条件的改变来改变细胞色素P450 BM3变体M13对染料木黄酮的区域选择性。
ACS Omega. 2020 Dec 2;5(49):32059-32066. doi: 10.1021/acsomega.0c05088. eCollection 2020 Dec 15.
7
Migratory birds as the potential source for the transmission of and other fungus to Bangladesh.候鸟作为向孟加拉国传播[具体内容缺失]和其他真菌的潜在源头。
J Adv Vet Anim Res. 2020 May 7;7(2):338-344. doi: 10.5455/javar.2020.g427. eCollection 2020 Jun.
8
Insight into metabolic diversity of the brown-rot basidiomycete Postia placenta responsible for sesquiterpene biosynthesis: semi-comprehensive screening of cytochrome P450 monooxygenase involved in protoilludene metabolism.深入了解负责倍半萜生物合成的褐腐担子菌胎盘 Postia 的代谢多样性：涉及原莪术烯代谢的细胞色素 P450 单加氧酶的半综合筛选。
Microb Biotechnol. 2018 Sep;11(5):952-965. doi: 10.1111/1751-7915.13304. Epub 2018 Aug 13.
9
Recombinant production of eukaryotic cytochrome P450s in microbial cell factories.真核细胞色素 P450s 在微生物细胞工厂中的重组生产。
Biosci Rep. 2018 Mar 5;38(2). doi: 10.1042/BSR20171290. Print 2018 Apr 27.
10
Structural insights into the mechanism of the drastic changes in enzymatic activity of the cytochrome P450 vitamin D hydroxylase (CYP107BR1) caused by a mutation distant from the active site.对细胞色素P450维生素D羟化酶（CYP107BR1）活性位点附近突变导致酶活性剧烈变化机制的结构洞察。
Acta Crystallogr F Struct Biol Commun. 2017 May 1;73(Pt 5):266-275. doi: 10.1107/S2053230X17004782. Epub 2017 Apr 26.
本文引用的文献
1
Potent Antioxidative Isoflavones Isolated from Soybeans Fermented with Aspergillus saitoi.从斋藤曲霉发酵大豆中分离出的强效抗氧化异黄酮。
Biosci Biotechnol Biochem. 1998;62(4):740-6. doi: 10.1271/bbb.62.740.
2
Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (sterol 14α-demethylase) doxycycline-regulated mutant and screening of the azole sensitivity of Aspergillus fumigatus isoenzymes CYP51A and CYP51B.酿酒酵母 ERG11/CYP51（甾醇 14α-脱甲基酶）强力霉素调控突变体的互补作用和烟曲霉同工酶 CYP51A 和 CYP51B 的唑类敏感性筛选。
Antimicrob Agents Chemother. 2010 Nov;54(11):4920-3. doi: 10.1128/AAC.00349-10. Epub 2010 Aug 23.
3
Cytochrome P450 monooxygenases involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium.细胞色素 P450 单加氧酶参与白腐真菌糙皮侧耳对蒽的代谢。
Appl Microbiol Biotechnol. 2010 Aug;87(5):1907-16. doi: 10.1007/s00253-010-2616-1. Epub 2010 May 28.
4
LICRED: a versatile drop-in vector for rapid generation of redox-self-sufficient cytochrome P450s.LICRED：一种通用的插入载体，可快速生成氧化还原自足细胞色素 P450。
Chembiochem. 2010 May 3;11(7):987-94. doi: 10.1002/cbic.201000104.
5
A versatile microbial system for biosynthesis of novel polyphenols with altered estrogen receptor binding activity.一种用于生物合成具有改变的雌激素受体结合活性的新型多酚的多功能微生物系统。
Chem Biol. 2010 Apr 23;17(4):392-401. doi: 10.1016/j.chembiol.2010.03.010.
6
Molecular characterization and isolation of cytochrome P450 genes from the filamentous fungus Aspergillus oryzae.从丝状真菌米曲霉中细胞色素 P450 基因的分子特征描述和分离。
Arch Microbiol. 2010 May;192(5):395-408. doi: 10.1007/s00203-010-0562-z. Epub 2010 Apr 1.
7
The cytochrome p450 homepage.细胞色素 p450 主页。
Hum Genomics. 2009 Oct;4(1):59-65. doi: 10.1186/1479-7364-4-1-59.
8
Molecular cloning and functional characterization of two CYP619 cytochrome P450s involved in biosynthesis of patulin in Aspergillus clavatus.与棒曲霉中展青霉素生物合成相关的两种CYP619细胞色素P450的分子克隆及功能表征
Microbiology (Reading). 2009 May;155(Pt 5):1738-1747. doi: 10.1099/mic.0.024836-0. Epub 2009 Apr 21.
9
Engineering cytochrome p450 enzymes.工程化细胞色素P450酶
Chem Res Toxicol. 2008 Jan;21(1):220-31. doi: 10.1021/tx7002849. Epub 2007 Dec 8.
10
CFGP: a web-based, comparative fungal genomics platform.CFGP：一个基于网络的比较真菌基因组学平台。
Nucleic Acids Res. 2008 Jan;36(Database issue):D562-71. doi: 10.1093/nar/gkm758. Epub 2007 Oct 18.
Zotero 插件