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Potent Antioxidative Isoflavones Isolated from Soybeans Fermented with Aspergillus saitoi.从斋藤曲霉发酵大豆中分离出的强效抗氧化异黄酮。
Biosci Biotechnol Biochem. 1998;62(4):740-6. doi: 10.1271/bbb.62.740.
2
Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (sterol 14α-demethylase) doxycycline-regulated mutant and screening of the azole sensitivity of Aspergillus fumigatus isoenzymes CYP51A and CYP51B.酿酒酵母 ERG11/CYP51（甾醇 14α-脱甲基酶）强力霉素调控突变体的互补作用和烟曲霉同工酶 CYP51A 和 CYP51B 的唑类敏感性筛选。
Antimicrob Agents Chemother. 2010 Nov;54(11):4920-3. doi: 10.1128/AAC.00349-10. Epub 2010 Aug 23.
3
Cytochrome P450 monooxygenases involved in anthracene metabolism by the white-rot basidiomycete Phanerochaete chrysosporium.细胞色素 P450 单加氧酶参与白腐真菌糙皮侧耳对蒽的代谢。
Appl Microbiol Biotechnol. 2010 Aug;87(5):1907-16. doi: 10.1007/s00253-010-2616-1. Epub 2010 May 28.
4
LICRED: a versatile drop-in vector for rapid generation of redox-self-sufficient cytochrome P450s.LICRED：一种通用的插入载体，可快速生成氧化还原自足细胞色素 P450。
Chembiochem. 2010 May 3;11(7):987-94. doi: 10.1002/cbic.201000104.
5
A versatile microbial system for biosynthesis of novel polyphenols with altered estrogen receptor binding activity.一种用于生物合成具有改变的雌激素受体结合活性的新型多酚的多功能微生物系统。
Chem Biol. 2010 Apr 23;17(4):392-401. doi: 10.1016/j.chembiol.2010.03.010.
6
Molecular characterization and isolation of cytochrome P450 genes from the filamentous fungus Aspergillus oryzae.从丝状真菌米曲霉中细胞色素 P450 基因的分子特征描述和分离。
Arch Microbiol. 2010 May;192(5):395-408. doi: 10.1007/s00203-010-0562-z. Epub 2010 Apr 1.
7
The cytochrome p450 homepage.细胞色素 p450 主页。
Hum Genomics. 2009 Oct;4(1):59-65. doi: 10.1186/1479-7364-4-1-59.
8
Molecular cloning and functional characterization of two CYP619 cytochrome P450s involved in biosynthesis of patulin in Aspergillus clavatus.与棒曲霉中展青霉素生物合成相关的两种CYP619细胞色素P450的分子克隆及功能表征
Microbiology (Reading). 2009 May;155(Pt 5):1738-1747. doi: 10.1099/mic.0.024836-0. Epub 2009 Apr 21.
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Engineering cytochrome p450 enzymes.工程化细胞色素P450酶
Chem Res Toxicol. 2008 Jan;21(1):220-31. doi: 10.1021/tx7002849. Epub 2007 Dec 8.
10
CFGP: a web-based, comparative fungal genomics platform.CFGP：一个基于网络的比较真菌基因组学平台。
Nucleic Acids Res. 2008 Jan;36(Database issue):D562-71. doi: 10.1093/nar/gkm758. Epub 2007 Oct 18.

构建和应用米曲霉细胞色素 P450 单加氧酶功能文库。

Construction and application of a functional library of cytochrome P450 monooxygenases from the filamentous fungus Aspergillus oryzae.

机构信息

Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.

出版信息

Appl Environ Microbiol. 2011 May;77(9):3147-50. doi: 10.1128/AEM.02491-10. Epub 2011 Mar 4.

DOI:10.1128/AEM.02491-10
PMID:21378053
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3126401/
Abstract

A functional library of cytochrome P450 monooxygenases from Aspergillus oryzae (AoCYPs) was constructed in which 121 isoforms were coexpressed with yeast NADPH-cytochrome P450 oxidoreductase in Saccharomyces cerevisiae. Using this functional library, novel catalytic functions of AoCYPs, such as catalytic potentials of CYP57B3 against genistein, were elucidated for the first time. Comprehensive functional screening promises rapid characterization of catalytic potentials and utility of AoCYPs.

摘要

构建了来自米曲霉（Aspergillus oryzae）的细胞色素 P450 单加氧酶的功能性文库，其中 121 种同工型与酵母 NADPH-细胞色素 P450 氧化还原酶在酿酒酵母中共同表达。利用这个功能性文库，首次阐明了 AoCYPs 的新催化功能，如 CYP57B3 对染料木黄酮的催化潜力。全面的功能筛选有望快速表征 AoCYPs 的催化潜力和用途。